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Expression Profiles of Planarain Stem Cells and their Immediate Division Progeny

ABSTRACT: Throughout adulthood, the replacement of cells lost to physiological turnover or injury in planarians is sustained by the proliferation and differentiation of adult stem cells known as neoblasts. Because neoblasts are the only known mitotic cells in asexual planarians, they can be efficiently eliminated by irradiation without significantly affecting post-mitotic differentiated cells. We uncovered a cohort of transcripts specific to the stem cells and their division progeny by defining the expression profiles of wild type and irradiated animals at different time points after irradiation. RNA from wild type animals, or from animals 24 hours or seven days after exposure to radiation, were compared to a common reference.

ORGANISM(S): Schmidtea mediterranea

SUBMITTER: Alejandro Sánchez Alvarado

PROVIDER: E-GEOD-11503 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular analysis of stem cells and their descendants during cell turnover and regeneration in the planarian Schmidtea mediterranea.

Eisenhoffer George T GT Kang Hara H Sánchez Alvarado Alejandro A

Cell stem cell 20080901 3

In adult planarians, the replacement of cells lost to physiological turnover or injury is sustained by the proliferation and differentiation of stem cells known as neoblasts. Neoblast lineage relationships and the molecular changes that take place during differentiation into the appropriate cell types are poorly understood. Here we report the identification and characterization of a cohort of genes specifically expressed in neoblasts and their descendants. We find that genes with severely downre ...[more]

PMID: 18786419

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