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Expression QTL mapping of Toxoplasma gondii genes

ABSTRACT: This SuperSeries is composed of the following subset Series: GSE11437: Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array GSE11514: Expression QTL mapping of Toxoplasma gondii genes, Tachyzoite array Keywords: SuperSeries Refer to individual Series

ORGANISM(S): synthetic construct

SUBMITTER: Jon Boyle

PROVIDER: E-GEOD-11515 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression quantitative trait locus mapping of toxoplasma genes reveals multiple mechanisms for strain-specific differences in gene expression.

Boyle Jon P JP Saeij Jeroen P J JP Harada Scott Y SY Ajioka Jim W JW Boothroyd John C JC

Eukaryotic cell 20080613 8

Toxoplasma gondii is an intracellular parasite with a significant impact on human health, especially in cases where individuals are immunocompromised (e.g., due to human immunodeficiency virus/AIDS). In Europe and North America, only a few clonal genotypes appear to be responsible for the vast majority of Toxoplasma infections, and these clonotypes have been intensely studied to identify strain-specific phenotypes that may play a role in the manifestation of more-severe disease. To identify and ...[more]

PMID: 18552283

Similar Datasets

Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array

Project description:Toxoplasma gondii is an intracellular parasite with a significant impact on human health, especially in cases where individuals are immunocompromised (e.g., due to HIV/AIDS). In Europe and North America only a few clonal genotypes appear to be responsible for the vast majority of Toxoplasma infections, and these clonotypes have been intensely studied to identify strain-specific phenotypes that may play a role in the manifestation of more severe disease. To identify and genetically map strain-specific differences in gene expression, we have carried out expression quantitative trait locus (eQTL) analysis on Toxoplasma gene expression phenotypes using spotted cDNA microarrays. This led to the identification of 16 Toxoplasma genes that had significant and mappable strain-specific variation in hybridization intensity. While the analysis should identify both cis and trans-mapping hybridization profiles, we only identified loci with strain-specific hybridization differences that are most likely due to differences in the locus itself (i.e., cis-mapping). Interestingly, a larger number of these cis-mapping genes than would be expected by chance encode either confirmed or predicted secreted proteins, many of which are known to localize to the specialized secretory organelles characteristic of members of the phylum Apicomplexa. For 6 of the cis-mapping loci we determined if the strain-specific hybridization differences were due to true transcriptional differences or rather strain-specific differences in hybridization efficiency because of extreme polymorphism and/or deletion, and we found examples of both scenarios. Keywords: eQTL mapping; virulence; Toxoplasma gondii 17 F1 progeny from a cross between a type II parent (PDS) and a type III parent (CTG) were used in RNA hybridizations to identify cis and trans-mapping loci regulating gene expression

2008-05-22 | E-GEOD-11437 | biostudies-arrayexpress

Expression QTL mapping of Toxoplasma gondii genes, Tachyzoite array

Project description:Toxoplasma gondii is an intracellular parasite with a significant impact on human health, especially in cases where individuals are immunocompromised (e.g., due to HIV/AIDS). In Europe and North America only a few clonal genotypes appear to be responsible for the vast majority of Toxoplasma infections, and these clonotypes have been intensely studied to identify strain-specific phenotypes that may play a role in the manifestation of more severe disease. To identify and genetically map strain-specific differences in gene expression, we have carried out expression quantitative trait locus (eQTL) analysis on Toxoplasma gene expression phenotypes using spotted cDNA microarrays. This led to the identification of 16 Toxoplasma genes that had significant and mappable strain-specific variation in hybridization intensity. While the analysis should identify both cis and trans-mapping hybridization profiles, we only identified loci with strain-specific hybridization differences that are most likely due to differences in the locus itself (i.e., cis-mapping). Interestingly, a larger number of these cis-mapping genes than would be expected by chance encode either confirmed or predicted secreted proteins, many of which are known to localize to the specialized secretory organelles characteristic of members of the phylum Apicomplexa. For 6 of the cis-mapping loci we determined if the strain-specific hybridization differences were due to true transcriptional differences or rather strain-specific differences in hybridization efficiency because of extreme polymorphism and/or deletion, and we found examples of both scenarios. Keywords: eQTL mapping; virulence; Toxoplasma gondii 19 F1 progeny from a cross between a type II parent (PDS) and a type III parent (CTG) were used in RNA hybridizations to identify cis and trans-mapping loci regulating gene expression

2008-05-22 | E-GEOD-11514 | biostudies-arrayexpress

Expression and genomic hybridization data for the Toxoplasma gondii I X III cross

Project description:This SuperSeries is composed of the following subset Series: GSE26558: Expression Quantitative Trait Locus (eQTL) Mapping of Stage-specific Gene Expression in Progeny from a type I X III Genetic Cross of Toxoplasma gondii GSE26607: Genomic hybridizations for the parents and progeny of the Toxoplasma gondii I X III genetic cross Refer to individual Series

2012-01-11 | E-GEOD-26608 | biostudies-arrayexpress

ROP16-dependent host transcriptional responses in chicken embryonic fibroblasts infected with T. gondii [RH, ROP16-KO]

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2011-05-27 | E-GEOD-29564 | biostudies-arrayexpress

Project description:Expression QTL mapping of Toxoplasma gondii genes

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Single-cell RNA-Seq of IFN-γ stimulated murine bone marrow-derived macrophages (BMDMs) infected Toxoplasma

Project description:To study the effect of stress on macrophages due to Toxoplasma, we stimulated murine bone marrow-derived macrophages (BMDMs) with IFN-γ (no-stimulate control) and infected them with the apicomplexan parasite Toxoplasma gondii. scRNA-Seq (10X Chromium genomics ) was performed to understand the changes in the immune cells and study the impact of the parasite.

2024-10-09 | E-MTAB-14453 | biostudies-arrayexpress

Toxoplasma gondii RNASeq

Project description:Recent advances in high throughput sequencing methodologies allow the opportunity to probe in depth the transcriptomes of organisms including N. caninum and Toxoplasma gondii. In this project, we are using Illumina sequencing technology to analyze the transcriptome (RNA-Seq) of experimentally accessible stages (e.g. tachyzoites at different times points) of T. gondii VEG strain. The aim is to make comparative transcriptional landscape maps of Neospora and Toxoplasma at different time points at different life cycle stages and compare levels of expression of orthologous genes in these two organisms.

2011-02-08 | E-MTAB-550 | biostudies-arrayexpress

Host responses in chicken embryonic fibroblasts infected with different strains of Toxoplasma gondii [F1 progeny]

2011-05-27 | E-GEOD-29563 | biostudies-arrayexpress

Strain-dependent host responses in chicken embryonic fibroblasts infected with Toxoplasma gondii [ME49, CEP, and Mock]

2011-05-27 | E-GEOD-29562 | biostudies-arrayexpress

Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array

Project description:Expression QTL mapping of Toxoplasma gondii genes, Bradyzoite array

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