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Transcription profiling of mouse embryo-derived stromal cell lines

ABSTRACT: Hematopoiesis occurs in a microenviroenment in which stromal cells are prominent. Stromal cells have been shown to maintain stem cell behaviour of hematopoietic stem cells. We derived several different stromal cell lines from midgestation embryos which will, or will not maintain hemetopoietic stem cells in cultures. We used microarrays to detail the global programme of gene expression underlying maintenance and emergence of hematopoietic stem cells in cocultures with embryo-derived stromal cells. CEL files will become available on November 29, 2008 Experiment Overall Design: Embryo-derived stromal cell lines were grown to confluence and irradiated as described earlier (Oostendorp et al., Stem Cells. 2005;23:842:50). Normalized Gene expression of the three different cell lines was compared.

ORGANISM(S): Mus musculus

SUBMITTER: Robert Oostendorp 

PROVIDER: E-GEOD-11589 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Efficient hematopoietic differentiation of human embryonic stem cells on stromal cells derived from hematopoietic niches.

Ledran Maria H MH   Krassowska Anna A   Armstrong Lyle L   Dimmick Ian I   Renström Jonas J   Lang Roland R   Yung Sun S   Santibanez-Coref Mauro M   Dzierzak Elaine E   Stojkovic Miodrag M   Oostendorp Robert A J RA   Forrester Lesley L   Lako Majlinda M  

Cell stem cell 20080701 1

Hematopoietic stem cells derived from human embryonic stem cells (hESCs) could provide a therapeutic alternative to bone marrow transplants, but the efficiency of currently available derivation protocols is low. In this study, we investigated whether coculture with monolayers of cells derived from mouse AGM and fetal liver, or with stromal cell lines derived from these tissues, can enhance hESC hematopoietic differentiation. We found that under such conditions hESC-derived differentiating cells  ...[more]

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