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Transcription profiling of chicken B cell lymphoma (DT40) cell lines.

ABSTRACT: Global gene expression profiling of the avian B-lymphoma DT40 cell line was used as a model to differentiate among Btk KO and Btk KO cells reconstituted with human Btk. Differences in the gene expression pattern showed statistically significant changes between parental DT40 and all the Btk KO cell populations irrespective of whether they are reconstituted or not. These results imply that in the process of generating a knockout cell line, subclones are selected, which have multiple changes in their gene expression pattern (p<0.01). Experiment Overall Design: DT40 cells along with the mutants were grown at various time points in different batches of fetal calf serum with or without any stimulation. All experiments were repeated ten times and then polled together as one sample. In total 28 samples were used RNA extraction and hybridization on Affymetrix microarrays.

ORGANISM(S): Gallus gallus

SUBMITTER: Hossain Nawaz 

PROVIDER: E-GEOD-11597 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Expression profiling of chicken DT40 lymphoma cells indicates clonal selection of knockout and gene reconstituted cells.

Nawaz Hossain M HM   Blomberg K Emelie M KEM   Lindvall Jessica M JM   Kurosaki Tomohiro T   Smith C I Edvard CIE  

Biochemical and biophysical research communications 20081016 2

The DT40 cell-line has been extensively used to create deletion mutants, one of which lacks Bruton's tyrosine kinase (Btk). Btk is a cytoplasmic tyrosine kinase important for B-lymphocyte maturation. It was previously shown that there are differences in gene expression between wild-type and Btk-deficient animals. Global gene expression profiling of the avian B-lymphoma DT40 cell-line was used as a model to differentiate among Btk knockout (KO) and Btk KO cells reconstituted with human Btk. Diffe  ...[more]

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