Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling by array of human MCF-7 breast cancer cells

ABSTRACT: Estrogen-responsive genes were identified by transcript profiling of estrogen-treated MCF-7 breast cancer cells. The gene expression profile generated after estrogen treatment was compared with that following inducible expression of c-Myc or c-Zip (a deletion mutant of c-Myc that lacks the N-terminal transactivation domains) in clonal MCF-7 cell lines. Experiment Overall Design: RNA was collected in three independent experiments, each including parental MCF-7 cells treated with 17b-estradiol (E2) or ethanol (EtOH), zinc-treated p-delta-MT-c-Myc cells, zinc-treated p-delta-MT-c-Zip cells and zinc-treated empty vector (p-delta-MT) cells. Cells were arrested for 48 h with 10 nM ICI 182780 and then treated for 6 h with either 100 nM E2 or ethanol vehicle, or 75 mM zinc for the stably transfected cell lines.

ORGANISM(S): Homo sapiens

SUBMITTER: Liz Musgrove 

PROVIDER: E-GEOD-11791 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Identification of functional networks of estrogen- and c-Myc-responsive genes and their relationship to response to tamoxifen therapy in breast cancer.

Musgrove Elizabeth A EA   Sergio C Marcelo CM   Loi Sherene S   Inman Claire K CK   Anderson Luke R LR   Alles M Chehani MC   Pinese Mark M   Caldon C Elizabeth CE   Schütte Judith J   Gardiner-Garden Margaret M   Ormandy Christopher J CJ   McArthur Grant G   Butt Alison J AJ   Sutherland Robert L RL  

PloS one 20080820 8

<h4>Background</h4>Estrogen is a pivotal regulator of cell proliferation in the normal breast and breast cancer. Endocrine therapies targeting the estrogen receptor are effective in breast cancer, but their success is limited by intrinsic and acquired resistance.<h4>Methodology/principal findings</h4>With the goal of gaining mechanistic insights into estrogen action and endocrine resistance, we classified estrogen-regulated genes by function, and determined the relationship between functionally-  ...[more]

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