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Growth of B. thetaiotaomicron on purified host mucosal glycans and glycan fragments

ABSTRACT: Bacteroides thetaiotaomicron was grown and transcriptionally profiled on a number of different host mucosal glycans and their component mono- and disaccharides. Experiments are performed in biological duplicate (2 replicates per growth condition). All cultures were harvested at similar points in mid-log phase of growth in minimal medium plus 0.5% (total amount, w/v) of a defined carbon source. Cultures on individual carbon sources are designed to be referenced to a minimal medium glucose control (GSM302686 and 302791) grown in the same growth format. Note that the hybridization targets used for these two reference datasets are from the exact same prep used for similar targets on the GPL1821 platform (sets GSM301720 and 301721)

ORGANISM(S): Bacteroides thetaiotaomicron

SUBMITTER: Eric Martens

PROVIDER: E-GEOD-11962 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mucosal glycan foraging enhances fitness and transmission of a saccharolytic human gut bacterial symbiont.

Martens Eric C EC Chiang Herbert C HC Gordon Jeffrey I JI

Cell host & microbe 20081101 5

The distal human gut is a microbial bioreactor that digests complex carbohydrates. The strategies evolved by gut microbes to sense and process diverse glycans have important implications for the assembly and operation of this ecosystem. The human gut-derived bacterium Bacteroides thetaiotaomicron forages on both host and dietary glycans. Its ability to target these substrates resides in 88 polysaccharide utilization loci (PULs), encompassing 18% of its genome. Whole genome transcriptional profil ...[more]

PMID: 18996345

Similar Datasets

Growth of B. thetaiotaomicron on purified host mucosal glycans and glycan fragments

Project description:Bacteroides thetaiotaomicron was grown and transcriptionally profiled on a number of different host mucosal glycans and their component mono- and disaccharides.

2008-11-21 | GSE11962 | GEO

Mucosal Glycan Foraging Enhances the Fitness and Transmission of a Saccharolytic Human Distal Gut Symbiont

Project description:Gene expression profiles of Bacteroides thetaiotaomicron in vitro during growth on host mucosal polysaccharides as sole carbon sources. All substrates in this series are derived from porcine gastric mucin and include mucin O-glycans and glycosaminoglycans. Two different culture formats used: 800ml batch-culture bioreactors and 5ml tube cultures (format is indicated within each sample title). Each set of growths was referenced to a minimal medium glucose reference corressponding to the same culture format. Unfractionated porcine mucosal glycan (PMG) growths were compared to previously published in vivo datasets, which were referenced to the 800ml minimal medium glucose reference dataset.

2008-11-20 | E-GEOD-11944 | biostudies-arrayexpress

Glycan effect on Saccharolytic Human Distal Gut Symbiont, B. thetaiotaomicron

Project description:This SuperSeries is composed of the following subset Series: GSE11944: Mucosal Glycan Foraging Enhances the Fitness and Transmission of a Saccharolytic Human Distal Gut Symbiont GSE11953: Mucosal Glycan Foraging Enhances the Fitness and Transmission of a Saccharolytic Human Distal Gut Symbiont: ECF mutant GSE11962: Growth of B. thetaiotaomicron on purified host mucosal glycans and glycan fragments Refer to individual Series

2008-11-20 | E-GEOD-11980 | biostudies-arrayexpress

Depolymerization of plant cell wall glycans by symbiotic human gut bacteria (Bacteroides thetaiotaomicron)

Project description:Symbiotic bacteria inhabiting the distal human gut have evolved under intense pressure to utilize complex carbohydrates, predominantly plant cell wall glycans abundant in our diets. These substrates are recalcitrant to depolymerization by digestive enzymes encoded in the human genome, but are efficiently targeted by some of the ~103-104 bacterial species that inhabit this niche. These species augment our comparatively narrow carbohydrate digestive capacity by unlocking otherwise unusable sugars and fermenting them into host-absorbable forms, such as short-chain fatty acids. We used phenotype profiling, whole-genome transcriptional analysis and molecular genetic approaches to investigate complex glycan utilization by two fully sequenced and closely related human gut symbionts: Bacteroides thetaiotaomicron and Bacteroides ovatus. Together these species target all of the common glycosidic linkages found in the plant cell wall, as well as host polysaccharides, but each species exhibits a unique ‘glycan niche’: in vitro B. thetaiotaomicron targets plant cell wall pectins in addition to linkages contained in host N- and O-glycans; B. ovatus uniquely targets hemicellulosic polysaccharides along with several pectins, but is deficient in host glycan utilization. Growth of Bacteroides thetaiotaomicron in vitro in minimal medium plus different purified complex glycans. Observation of increased gene expression was used to determine genes that are involved in metabolism of each glycan. Two biological replicates each.

2011-05-01 | E-GEOD-25572 | biostudies-arrayexpress

Analysis of the transcriptome of Bacteroides thetaiotaomicron during growth on complex N-glycan as the sole carbon source

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2019-04-26 | GSE129572 | GEO

RNAseq of the human gut commensal Bacteroides thetaiotaomicron wildtype, bt0700 and bt0700 bt3998 (ppGpp0) strains across three conditions: glucose minimal medium, 1h of carbon starvation, and mouse gut colonization

Project description:Transcriptional profiles of wildtype and mutant strains bt0700 and bt0700 bt3998 (ppGpp0) of human gut commensal B. thetaiotaomicron were collected to investigate the relevance of the stringent response mediated by the alarmone (p)ppGpp for growth and survival in three different conditions. RNAseq samples were taken during growth in glucose minimal medium, after 1h of carbon starvation, and after 6 days of monocolonizing germ-free mice (from cecum material).

2018-07-04 | E-MTAB-6412 | biostudies-arrayexpress

Coordinate regulation of glycan degradation and polysaccharide capsule biosynthesis by a prominent human gut symbiont

Project description:Analysis of B. thetaiotaomicron trans-envelope signaling mutants in ECF-sigma factors, anti-sigma factors and SusC-like transporters. B. thetaiotaomicron mutants were transcriptionally profiled after growth in vitro in either minimal medium plus glucose or minimal medium plus neutral O-glycans

2010-05-18 | E-GEOD-14592 | biostudies-arrayexpress

Regulated expression of polysaccharide utilization and capsular biosynthesis loci in biofilm and planktonic Bacteroides thetaiotaomicron during growth in chemostats

Project description:We used Affymetrix GeneChips to determine the physiological differences between biofilm and planktonic cells of Bacteroides thetaiotaomicron strain VPI-5482 (ATCC 29148) by comparing gene expression. For this purpose, B. thetaiotaomicron cells were grown in sterile, continuous flow bioreactors fed with tryptone, yeast extract, glucose (TYG) medium. The bioreactors were controlled at a temperature of 37C using a water jacket and a recirculating water heater. After 8 hours post-inoculation, planktonic cells were harvested from the bulk solution in the bioreactor, and after 8 days post-inoculation, the biofilm was scraped from the carbon paper. RNA was harvested from both biofilm and planktonic populations. RNA was extracted by a phenol:chloroform method and purified with a Qiagen RNA Easy mini-kit. Overall growth conditions are summarized above. The experimental conditions were: biofilm (BF), and planktonic (PL).

2014-01-01 | E-GEOD-38534 | biostudies-arrayexpress

B. thetaiotaomicron or B. longum mono-association vs. bi-association

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2010-07-01 | E-GEOD-5869 | biostudies-arrayexpress