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Analysis of small RNAs in murine neutrophils by Solexa/Illumina genome analyzer

ABSTRACT: To examine the effects of microRNAs including miR-223 on murine neutrophil function, small RNAs cloning and sequencing from neutrophils were performed. Neutrophils were isolated from a wild-type mouse and total RNAs were prepared. Small RNAs were cloned and sequenced by Solexa/Illumina genome analyzer.

ORGANISM(S): Mus musculus

SUBMITTER: Whitehead Institute

PROVIDER: E-GEOD-12074 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The impact of microRNAs on protein output.

Baek Daehyun D Villén Judit J Shin Chanseok C Camargo Fernando D FD Gygi Steven P SP Bartel David P DP

Nature 20080730 7209

MicroRNAs are endogenous approximately 23-nucleotide RNAs that can pair to sites in the messenger RNAs of protein-coding genes to downregulate the expression from these messages. MicroRNAs are known to influence the evolution and stability of many mRNAs, but their global impact on protein output had not been examined. Here we use quantitative mass spectrometry to measure the response of thousands of proteins after introducing microRNAs into cultured cells and after deleting mir-223 in mouse neut ...[more]

PMID: 18668037

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