Unknown,Transcriptomics,Genomics,Proteomics

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Listeria monocytogenes EGD after growth BHI vs. LB vs. MM


ABSTRACT: Transcriptional profile of prfA, the dependent genes and the PTS genes after growth in BHI (brain-heart-infusion), LB (Luria Bertani broth) and MM (minimal medium (Premaratne et al. 2001) (each supplemented with 50 mM Glucose). Keywords: media comparison A total of three independently isolated RNA samples from each condition were used for the analysis.

ORGANISM(S): Listeria monocytogenes

SUBMITTER: Regina Stoll 

PROVIDER: E-GEOD-12143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Modulation of PrfA activity in Listeria monocytogenes upon growth in different culture media.

Stoll Regina R   Mertins Sonja S   Joseph Biju B   Müller-Altrock Stefanie S   Goebel Werner W  

Microbiology (Reading, England) 20081201 Pt 12


PrfA is the major transcriptional activator of most virulence genes of Listeria monocytogenes. Its activity is modulated by a variety of culture conditions. Here, we studied the PrfA activity in the L. monocytogenes wild-type strain EGD and an isogenic prfA deletion mutant (EGDDeltaprfA) carrying multiple copies of the wild-type prfA or the mutant prfA* gene (strains EGDDeltaprfApPrfA and EGDDeltaprfApPrfA*) in response to growth in brain heart infusion (BHI), Luria-Bertani broth (LB) or a defin  ...[more]

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