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Transcription profiling of human samples after exercise training to characterize a colony assay used to investigate endothelial progenitor cells


ABSTRACT: Subjects underwent endothelial testing and blood sampling at baseline and after 3 months of exercise training. Microarray and flow cytometry-based characterization of cells from an endothelial progeniator colony assay was consistent with T lymphocytes, but not endothelial cells. Experiment Overall Design: blood samples from 12 healthy subjects at baseline (A) and after 3 months (B) of exercise.

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Cannon 

PROVIDER: E-GEOD-12155 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Microarray-based characterization of a colony assay used to investigate endothelial progenitor cells and relevance to endothelial function in humans.

Desai Aditi A   Glaser Alexander A   Liu Delong D   Raghavachari Nalini N   Blum Arnon A   Zalos Gloria G   Lippincott Margaret M   McCoy J Philip JP   Munson Peter J PJ   Solomon Michael A MA   Danner Robert L RL   Cannon Richard O RO  

Arteriosclerosis, thrombosis, and vascular biology 20090101 1


<h4>Objective</h4>An assay proposed to quantify endothelial progenitor cell (EPC) colonies in humans was investigated to determine the phenotype of recovered cells and their relevance to in vivo endothelial function.<h4>Methods and results</h4>Twelve sedentary subjects participating in a worksite wellness program underwent endothelial flow-mediated dilation (FMD) testing of the brachial artery and blood sampling for EPC colony assay. Microarray-based genotypic characterization of colonies showed  ...[more]

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