Transcriptional profile of pmt1 pmt2 mutants
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: Javier Arroyo
PROVIDER: E-GEOD-12181 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Similar Datasets
Project description:We did transcription profiling on the effect of pmt1 pmt4 deletion, genes involved in the O-glycosylation process. These mutants cells show inhibition of mating, filamentation and induction of cell wall compensatory mechanism. Two biological samples were analyzed for each condition, and a microarray experiment were carried out for each sample 1. wild type cells SEY6210 2. pmt1 pmt2 cells from SEY6210 background
2010-09-29 | E-GEOD-12182 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE12180: Wild type 3 hours exposure to 1 microM OGT2468 GSE12181: Transcriptional profile of pmt1 pmt2 mutants GSE12182: Transcriptional profile of pmt1 pmt4 mutants GSE12193: Wild type 3 hours exposure to 0.1 microM OGT2468 Refer to individual Series
2010-09-29 | E-GEOD-12197 | biostudies-arrayexpress
Project description:We did transcription profiling on the effect of rlm1 (MAPK Slt2 transcription factor) deletion and swi3 (component of SWI/SNF complex involved in chromatin remodeling) deletion in genes involved in cell wall stress (Congo Red) response. Three biological samples were analyzed for each condition, and a microarray experiment were carried out for each sample 1. absence of the drug 2. presence of 30 µg/ml of Congo Red
2012-05-24 | E-GEOD-31176 | biostudies-arrayexpress
Project description:We studied the transcriptional profile in response to acute PtdIns-4,5P2 depletion induced by heterologous expression of a plasma membrane-directed version of mammalian PI3K catalytic subunit (p110M-NM-1-CAAX). Three biological samples were analyzed for samples expressing for 4 hours p110M-NM-1-CAAX (PI3K) versus the kinase dead mutant p110M-NM-1-CAAX K802R (KD), and one microarray experiment was carried out for each sample.
2014-07-19 | E-GEOD-59596 | biostudies-arrayexpress
Project description:The availability of yeast DNA microarrays provides the possibility of monitoring gene expression levels as a function to toxin exposure, and consequently as a means of determining mechanisms of toxicity. This system possesses the benefit of the essential volume of yeast cultures, the high reproducibility of expression profiles, and the massive functional information. Because small amount of biological sample cultures are required for this analysis, toxicity test for rare chemical such as mycotoxins which is a natural compound and difficult to be artificially synthesized. Citrinin [518-75-2], 4,6-dihydro-8-hydroxy-3,4,5-trimethyl-6-oxo-3H-2-benzopyran-7-crboxylic acid, is the one of the popular mycotoxicin produced by Penicillium and Aspergillus family possibly spread all over the world. This natural chemicals is one of the well characterized mycotoxin but the information especially mechanism of toxic action is limited. We used two types of microarrays, one have ORF (Open reading Frame) fragment on the surface of the glass as probes and the other have probes as oligonucleotide probes on the microarray with 3 dimensions. We compared data properties and studied the toxicity of citrinin to yeast cells. Keywords: stress response Series containes 3 hybridization results from independent biological samples, and each experiment have high and low power scanned data respectively.
2010-06-10 | E-GEOD-6111 | biostudies-arrayexpress
Project description:The availability of yeast DNA microarrays provides the possibility of monitoring gene expression levels as a function to toxin exposure, and consequently as a means of determining mechanisms of toxicity. This system possesses the benefit of the essential volume of yeast cultures, the high reproducibility of expression profiles, and the massive functional information. Because small amount of biological sample cultures are required for this analysis, toxicity test for rare chemical such as mycotoxins which is a natural compound and difficult to be artificially synthesized. Citrinin [518-75-2], 4,6-dihydro-8-hydroxy-3,4,5-trimethyl-6-oxo-3H-2-benzopyran-7-crboxylic acid, is the one of the popular mycotoxicin produced by Penicillium and Aspergillus family possibly spread all over the world. This natural chemicals is one of the well characterized mycotoxin but the information especially mechanism of toxic action is limited. We used two types of microarrays, one have ORF (Open reading Frame) fragment on the surface of the glass as probes and the other have probes as oligonucleotide probes on the microarray with 3 dimensions. We compared data properties and studied the toxicity of citrinin to yeast cells. Keywords: stress response Biological samples were prepared from 3 independent experiments. Hybridization were done triplicate in each sample-control set. Sample labeling were swapped between 1st hybridization (control = Cy5, sample = Cy3) and 2nd and 3rd hybridization (control = Cy3, sample = Cy5).
2010-06-10 | E-GEOD-6101 | biostudies-arrayexpress
Project description:This SuperSeries is composed of the following subset Series: GSE6101: 300 ppm Citrinin treatment for 2 h (GPL4399) GSE6111: 300 ppm Citrinin treatment for 2 h (GPL1945) Keywords: SuperSeries Refer to individual Series
2010-06-10 | E-GEOD-6118 | biostudies-arrayexpress
Project description:We did transcription profiling on the effect of O-glycosylation inhibitor OGT2468 in Saccharomyces cerevisiae using strains SEY6210 (wild type). Yeast cells exposed to OGT2468 in YPD growth medium show a significant inhibition of mating, filamentation and induction of cell wall compensatory mechanism. Three biological samples were analyzed for each condition, and a microarray experiment were carried out for each sample 1. absence of the drug (but the corresponding amount of DMSO 0.1%) 2. presence of 0.1 microMolar of OGT2468
2010-09-29 | E-GEOD-12193 | biostudies-arrayexpress
Project description:We did transcription profiling on the effect of O-glycosylation inhibitor OGT2468 in Saccharomyces cerevisiae using strains SEY6210 (wild type). Yeast cells exposed to OGT2468 in YPD growth medium show a significant inhibition of mating, filamentation and induction of cell wall compensatory mechanism. Three biological samples were analyzed for each condition, and a microarray experiment were carried out for each sample 1. absence of the drug (but the corresponding amount of DMSO 1%) 2. presence of 1 microMolar of OGT2468
2010-09-29 | E-GEOD-12180 | biostudies-arrayexpress
Project description:Actin-related proteins are ubiquitous components of chromatin remodelers, and are conserved from yeast to man. We have examined the role of the budding yeast actin-related protein Arp6 in gene expression, both as a component of the SWR1 complex (SWR-C) and in its absence. We mapped Arp6-binding sites genome-wide using chromatin immunoprecipitation in mutant and wild-type cells. We find that the majority of Arp6-binding sites in euchromatin coincide with binding sites of Swr1, the catalytic subunit of SWR-C, and with the histone H2A variant Htz1. However, the remaining Arp6 binding in telomeres, centromeres, and the promoters of ribosomal protein (RP) genes are independent of Swr1 and Htz1 deposition. We show that Arp6 can position chromatin at nuclear pores, and is required for the pore association of the RP genes to which it binds. This anchoring is also independent of Swr1. Loss of Arp6, but not Htz1, leads to an up-regulation of RP genes and loss of relocalization. This is in contrast to the Htz1-mediated pore-association of GAL1, for which loss of Arp6 impairs activation. Given that Arp6 is required for SWR-C dependent deposition of Htz1, we conclude that Arp6 contributes to both H2AZ-dependent and H2AZ-independent association with nuclear pores and subsequent effects on gene expression. These data illustrate how nuclear actin-related proteins contribute to the long-range organization of chromatin domains in the interphase nucleus. Four replicates for the arp6 deletion mutant and three replicates for the swr1 deletion mutant compared to wild-type.
2010-05-01 | E-GEOD-17780 | biostudies-arrayexpress