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Analysis of growth-phase regulated genes in Streptococcus agalactiae by global transcript profiling

ABSTRACT: We determined dynamic behavior of S. agalactiae transcriptome during growth in THY medium and detected growth phase regulated genes Three independent cultures (three biological replicates) of S. agalactiae NEM316 strain in rich laboratory medium were sampled at mid log, late log early and late stationary growth phase.

ORGANISM(S): Streptococcus agalactiae

SUBMITTER: Izabela Sitkiewicz

PROVIDER: E-GEOD-12238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome adaptation of group B Streptococcus to growth in human amniotic fluid.

Sitkiewicz Izabela I Green Nicole M NM Guo Nina N Bongiovanni Ann Marie AM Witkin Steven S SS Musser James M JM

PloS one 20090701 7

<h4>Background</h4>Streptococcus agalactiae (group B Streptococcus) is a bacterial pathogen that causes severe intrauterine infections leading to fetal morbidity and mortality. The pathogenesis of GBS infection in this environment is poorly understood, in part because we lack a detailed understanding of the adaptation of this pathogen to growth in amniotic fluid. To address this knowledge deficit, we characterized the transcriptome of GBS grown in human amniotic fluid (AF) and compared it with t ...[more]

PMID: 19568429

Similar Datasets

Remodeling of the Streptococcus agalactiae transcriptome in response to growth temperature

Project description:To understand the extent to which GBS modify gene expression in response to temperatures encountered in the various hosts, we conducted a whole genome transcriptome analysis of organisms grown at 30°C and 40°C. We identified extensive transcriptome remodeling at various stages of growth, especially in the stationary phase (significant transcript changes occurred for 25% of the genes). A large proportion of genes involved in metabolism was up-regulated at 30°C in stationary phase, which reflects a slowing of bacterial metabolism in the early stages of its shift to growth at lower temperature followed by an acceleration in the later stages. Conversely, genes up-regulated at 40°C relative to 30°C include those encoding virulence factors such as hemolysins and extracellular secreted proteins with LPXTG motifs. Over-expression of hemolysins was linked to larger zones of hemolysis and enhanced hemolytic activity at 40°C. A key theme identified by our study was that genes involved in purine metabolism and iron acquisition were significantly up-regulated at 40°C. Three cultures of GBS grown in rich Todd-Hewitt Yeast extract medium at 30°C and three cultures grown at 40°C served as a source of RNA samples collected at mid-logarithmic phase, late-logarithmic phase, and stationary growth phase. RNA was isolated using a modified Trizol method, and targets for hybridization with custom Affymetrix chip were generated as we have described previously. Genes with 30°C/40°C ratio greater than 2 and less than 0.5 (P value less than 0.05), were considered differentially expressed.

2008-09-01 | E-GEOD-11666 | biostudies-arrayexpress

Transcriptome adaptation of group B Streptococcus to growth in human amniotic fluid

Project description:We have discovered that GBS significantly remodels its transcriptome in response to exposure to human amniotic fluid. A large number of the affected genes are of unknown function, which means that much remains to be learned about the full influence of amniotic fluid on GBS. The majority of the observed changes in transcripts affects genes involved in basic bacterial metabolism and is connected to AF composition and nutritional requirements of the bacterium. The observation that many genes encoding adhesions are down-regulated, and genes encoding known virulence factors such as a hemolysin and a potent IL-8 proteinase are up-regulated likely have consequences for the outcome of host-pathogen interactions. Streptococcus agalactiae, serotype III strain NEM316 was grown in human amniotic fluid. Samples of bacteria were collected in mid log, late log and stationary growth phases and were used for RNA isolation for microarray analysis

2010-05-18 | E-GEOD-14456 | biostudies-arrayexpress

Transcriptomic impact of the Rex deletion in Streptococcus agalactiae NEM 316 strain

Project description:Transcritpomic analysis of rex mutant (gbs1167) in NEM316 S. agalactiae strain in static and aerated growth condition

2020-04-10 | E-MTAB-8970 | biostudies-arrayexpress

First comprehensive proteome analysis of lysine crotonylation in Streptococcus agalactiae, a pathogen causing meningoencephalitis in teleosts

Project description:In Homo sapiens, Streptococcus agalactiae is a common colonizer of the rectovaginal tract and a fundamental cause of neonatal and non-pregnant adults infectious diseases. It also causes infectious disease in fish which compromises food safety as well as possesses a zoonotic risk. Lysine crotonylation (Kcr) is a type of histone post-translational modifications discovered in 2011. Kcr dynamics are involved in active gene promoters and potential enhancers in yeast and mammalian. However, lysine crotonylation in S. agalactiae has not yet been studied. In the present study, we conducted the first proteome-wide profiling of Kcr in S. agalactiae and identified 241 Kcr sites on 675 proteins, representing the Kcr event in S. agalactiae. Bioinformatics analysis showed that 164 sequences were matched to a total of six definitively conserved motifs, and many of them were significantly enriched in metabolic processes, cellular process, and single-organism processes. Moreover, we found four crotonylation modified proteins predicted as quorum sensing system and virulence factors, which indicate the important role of PTM on bacterial QS system and virulence. These data represent the first report of a global crotonylation proteome and provide a promising starting point for further functional research of crotonylation in bacterial virulence in S. agalactiae.

2021-12-30 | PXD026445 | Pride

rogB mutant in NEM316 S. agalactiae strain

Project description:transcritpomic analysis of rogB mutant in NEM316 S. agalactiae strain

2012-05-21 | E-MEXP-3237 | biostudies-arrayexpress

CovRS response in S. agalactiae NEM316 strain

Project description:Analysis of the CovRS response in S. agalactiae NEM316 strain with microarrays

2012-05-21 | E-MEXP-3304 | biostudies-arrayexpress

Comparison of gene expression in MGAS315 and its isogenc mtsR mutant

Project description:RATIONALE: mtsR is a regulator of genes involved in ion aquisition in S. pyogenes that has been suggested to be involved in development of invasive infections. We wanted to identify mtsR regulon. EXPERIMENT: We compared global gene expression in wild type strain MGAS315 with expression in its isogenc mtsR mutant. Experiment was performed with bacteria in exponential (EXP) and at late exponential phase (Late EXP) grown in THY medium Keywords: strain comparison 1. Three independent cultures of WT or mutant strain bacteria at exponential phase of growth (OD60=0.5, biological replicates) served as source of total RNA. RNA was transcribed into cDNA, fragmented and labeled using standard Affymetrix protocol for prokaryotic target preparation. Hybridization with custom Affymetrix array and scaning was performed using protocol recomended by manufacturer. Hybridizaton values aquired by GCOS (in CHP files) are normalized to global hybridization intesity. 2. Analogous experiment was performed with bacteria grown to OD600=1.2 representing late exponential growth phase

2010-05-06 | E-GEOD-10252 | biostudies-arrayexpress

rga response

Project description:comparison of the transcriptionnal response of the rga mutant and the wild type in S. agalactiae NEM316 strain

2012-05-21 | E-MEXP-3236 | biostudies-arrayexpress

Induction of adaptive changes in the Streptococcus agalactiae transcriptome by human blood

Project description:In the present investigation, we conducted a bacterial transcriptome dynamics analysis during GBS incubation with human blood. We observed a large modification in the expression of genes involved in transcriptional regulation and in the metabolism of carbohydrates, purins/pyrimidins and inorganic ions, and an up-regulation of genes involved in attachment and plasminogen contact. We also observed differences in the expression of GBS genes in blood at 40° relative to 37°C. The serotype III GBS reference strain NEM316 was cultivated in Todd Hewitt broth supplemented with 0.2% yeast extract (THY) in 5% CO2 at 37°C until OD reached 0.7. Culture was harvested by centrifugation 8 minutes at 4000 x g at 37°C, then suspended in phosphate-buffered saline (PBS). Fresh heparinized human blood was obtained from seven healthy voluntary donors (four males and three females) in accordance with a protocol approved by the Institutional Review Board of the Methodist Hospital Research Institute. The blood was maintained at 37°C no more than one hour prior to use. Bacterial suspension was then incubated with the blood of each donor at 37°C and 40°C under slight rotation to avoid sedimentation of blood and bacterial cells (the volume of blood was equivalent to the volume of bacterial culture prior to centrifugation). Samples were removed for microarrays analysis immediately after adding bacteria, and after 30 and 90 minutes of incubation. Thus, for each of the seven blood donors, we obtained results for five time points: immediately after mixing the bacterium with the blood (time 0), after 30 minutes of contact with blood (time 1) at 37°C and 40°C, and after 90 minutes of contact with blood (time 2) at 37°C and 40°C.

2008-10-31 | E-GEOD-11705 | biostudies-arrayexpress

Adaptation of group A Streptococcus to human amniotic fluid

Project description:Background. For more than 100 years, group A Streptococcus has been identified as a cause of severe, and in many cases fatal, infections of the female urogenital tract. Due to advances in hospital hygiene and the advent of antibiotics, this type of infection has been virtually eradicated. However, within the last three decades there has been an increase in severe intra- and post-partum infections attributed to GAS. Principal Findings. The majority of changes in the GAS transcriptome involved down regulation of multiple adhesins and virulence factors and activation of the stress response. We observed significant changes in genes involved in the arginine deiminase pathway and in the nucleotide de novo synthesis pathway. Conclusions/Significance. Our work provides new insight into how pathogenic bacteria respond to their environment to establish infection and cause disease. To identify genes that were up or down regulated in response to growth in AF, GAS was grown in human AF or standard laboratory media (THY) and samples for expression microarray analysis were collected during mid-logarithmic, late-logarithmic, and stationary growth phases. Microarray analysis was performed using a custom Affymetrix chip and normalized hybridization values derived from three biological replicates were collected at each growth point. Ratios of AF/THY above a 2-fold change and P-value < 0.05 were considered significant.

2010-05-05 | E-GEOD-19985 | biostudies-arrayexpress

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