Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of human primary breast tumors


ABSTRACT: Brain metastasis is one of the most feared complications of cancer and the most common intracranial malignancy in adults. Its underlying mechanisms remain unknown. From breast cancer patients with metastatic disease we isolated cell populations that aggressively colonize the brain. Transcriptomic analysis of these cells yielded overlapping gene sets whose expression is selectively associated with brain metastasis. The expression of seventeen of these genes in primary breast tumors is associated with brain relapse in breast cancer patients. Some of these genes are also associated with metastasis to lung but not to liver, bone or lymph nodes, providing a molecular basis for the long-observed link between brain and lung metastasis. Among the functionally validated brain metastasis genes, the cyclooxigenase COX-2, the EGFR ligand HB-EGF, and the brain-specific 2-6 sialyltransferase ST6GALNAC5 mediate cancer cell passage through the blood-brain barrier. Other brain metastasis genes encode inflammatory factors and brain-specific proteolytic regulators, suggesting a multifaceted program for breast cancer colonization of the brain. Experiment Overall Design: 204 primary tumors from breast cancer patients with known site of relapse were studied, focussing on brain relapse versus other relapse. Identified genes were validated in this cohort.

ORGANISM(S): Homo sapiens

SUBMITTER: Marcel Smid 

PROVIDER: E-GEOD-12276 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications


The molecular basis for breast cancer metastasis to the brain is largely unknown. Brain relapse typically occurs years after the removal of a breast tumour, suggesting that disseminated cancer cells must acquire specialized functions to take over this organ. Here we show that breast cancer metastasis to the brain involves mediators of extravasation through non-fenestrated capillaries, complemented by specific enhancers of blood-brain barrier crossing and brain colonization. We isolated cells tha  ...[more]

Similar Datasets

2009-05-06 | GSE12237 | GEO
2009-06-13 | GSE12276 | GEO
2009-05-16 | E-GEOD-12237 | biostudies-arrayexpress
2014-04-24 | E-GEOD-43837 | biostudies-arrayexpress
2020-05-26 | PXD010506 | Pride
2013-05-15 | E-GEOD-46928 | biostudies-arrayexpress
2017-05-23 | PXD005719 | Pride
2018-12-25 | GSE124344 | GEO
2011-06-20 | E-GEOD-26338 | biostudies-arrayexpress
2014-04-24 | GSE43837 | GEO