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Transcriptome profiling of maize anthers using genetic ablation to analyze pre-meiotic and tapetal cell types

ABSTRACT: Agilent oligonucleotide arrays were used to profile gene expression in dissected maize anthers of 3 types of male-sterile plants and their fertile siblings at four stages of development: after anther initiation, at the rapid mitotic proliferation stage, pre-meiosis, and meiotic prophase I. The male-sterile mutants (ms23, msca, and mac1) lack a range of normal cell types resulting from a temporal progression of anther failure. By combining the data sets from the comparisons between individual sterile and fertile anthers, candidate genes predicted to play important roles during maize anther development were assigned to stages and to likely cell types. Keywords: anther development, maize, male sterility 3 biological replicates of 3 different male-sterile mutants and their fertile siblings at 4 stages of anther development (36 arrays). Comparisons were done between male-sterile and fertile sibs at the same development stage.

ORGANISM(S): Zea mays

SUBMITTER: John Fernandes 

PROVIDER: E-GEOD-12756 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome profiling of maize anthers using genetic ablation to analyze pre-meiotic and tapetal cell types.

Ma Jiong J   Duncan David D   Morrow Darren J DJ   Fernandes John J   Walbot Virginia V  

The Plant journal : for cell and molecular biology 20070405 4

Oligonucleotide arrays were used to profile gene expression in dissected maize anthers at four stages: after-anther initiation, at the rapid mitotic proliferation stage, pre-meiosis, and meiotic prophase I. Nearly 9200 sense and antisense transcripts were detected, with the most diverse transcriptome present at the pre-meiotic stage. Three male-sterile mutants lacking a range of normal cell types resulting from a temporal progression of anther failure were compared with fertile siblings at equiv  ...[more]

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