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Control of Virulence Gene Expression in Citrobacter rodentium by Trascription Factor RegA

ABSTRACT: Identification of the targets of RegA with and without bicarbonate stimulation by comparing RegA knockout to multicopy RegA transgenics. RegA is an AraC like transcription factor identified in a mutational screen for virulence genes in Citrobacter rodentium, an attaching and effacing pathogen that causes transmissible colonic hyperplasia in mice. This experiment compares the RegA null strain with a multicopy plasmid rescue of this null strain in the presence and absence of bicarbonate with the aim of identifying pathogenesis related genes related to the early and late stages of attachment and effacement. Keywords: genetic modification, transcription factor, induction A strain of Citrobacter rodentium with a knockout of RegA was compared to the same strain rescued with a multicopy plasmid containing the wildtype RegA gene. These strains were analyzed with and without bicarbonate in an unconnected two factor design with dye balanced biological replicates.

ORGANISM(S): Citrobacter rodentium

SUBMITTER: Roy Robins-Browne 

PROVIDER: E-GEOD-12876 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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RegA, an AraC-like protein, is a global transcriptional regulator that controls virulence gene expression in Citrobacter rodentium.

Hart Emily E   Yang Ji J   Tauschek Marija M   Kelly Michelle M   Wakefield Matthew J MJ   Frankel Gad G   Hartland Elizabeth L EL   Robins-Browne Roy M RM  

Infection and immunity 20080902 11

Citrobacter rodentium is an attaching and effacing pathogen which causes transmissible colonic hyperplasia in mice. Infection with C. rodentium serves as a model for infection of humans with enteropathogenic and enterohemorrhagic Escherichia coli. To identify novel colonization factors of C. rodentium, we screened a signature-tagged mutant library of C. rodentium in mice. One noncolonizing mutant had a single transposon insertion in an open reading frame (ORF) which we designated regA because of  ...[more]

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