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Transcription profiling of mouse ovaries lacking Foxl2, Wnt4 or Kit and wild type ovaries and testes

ABSTRACT: Comparison of Foxl2-null ovaries to wildtype ovaries, ovaries lacking Wnt4 or Kit, or testes, throughout mouse development. The goal of this study was to identify early Foxl2 target genes as well as other ovarian, anti-testis genes that may act independently of Foxl2. Experiment Overall Design: We studied 43 samples over 15 conditions to cover a wide range of wiltype and pathological states showing highly divergent alterations of cell type composition. This was meant to identify the most specific, cell context-independent targets of Foxl2.

ORGANISM(S): Mus musculus

SUBMITTER: Chris Ottolenghi

PROVIDER: E-GEOD-12905 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Foxl2 functions in sex determination and histogenesis throughout mouse ovary development.

Garcia-Ortiz José Elias JE Pelosi Emanuele E Omari Shakib S Nedorezov Timur T Piao Yulan Y Karmazin Jesse J Uda Manuela M Cao Antonio A Cole Steve W SW Forabosco Antonino A Schlessinger David D Ottolenghi Chris C

BMC developmental biology 20090618

<h4>Background</h4>Partial loss of function of the transcription factor FOXL2 leads to premature ovarian failure in women. In animal models, Foxl2 is required for maintenance, and possibly induction, of female sex determination independently of other critical genes, e.g., Rspo1. Here we report expression profiling of mouse ovaries that lack Foxl2 alone or in combination with Wnt4 or Kit/c-Kit.<h4>Results</h4>Following Foxl2 loss, early testis genes (including Inhbb, Dhh, and Sox9) and several no ...[more]

PMID: 19538736

Similar Datasets

Foxl2 functions in sex determination and histogenesis throughout mouse ovary development, analyzed by Affymetrix arrays

Project description:Comparison of Foxl2-null ovaries to wildtype ovaries, ovaries lacking Wnt4 or Kit, or testes, throughout mouse development. The goal of this study was to identify early Foxl2 target genes as well as other ovarian, anti-testis genes that may act independently of Foxl2. Keywords: disease state analysis; genetic modification; developmental study

2009-06-24 | GSE12905 | GEO

Foxl2 functions in sex determination and histogenesis throughout mouse ovary development, analyzed by Agilent arrays

Project description:Partial loss of function of the transcription factor FOXL2 leads to premature ovarian failure in women. In animal models, Foxl2 is required for maintenance, and possibly induction, of female sex determination independently of other critical genes, i.e., Rspo1 and Wnt4. Here we report expression profiling of mouse ovaries that lack Foxl2 alone or in combination with Wnt4 or Kit/c-Kit, to identify ovarian targets of Foxl2 that, along with some testis genes, were dysregulated during embryonic development. Loss of one copy of Foxl2 revealed strong gene dosage sensitivity, with molecular anomalies that were milder but resembled ovaries lacking both Foxl2 alleles. Furthermore, a Foxl2 transgene disrupted embryonic testis differentiation and increased the levels of key female markers. The results, including a comprehensive principal component analysis of published microarray datasets 1) support the proposal of dose-dependent Foxl2 function and anti-testis action throughout ovary differentiation; and 2) identify candidate genes for a role in sex determination independent of FOXL2 (notably, the transcription factor, ZBTB7C) and in the generation of the ovarian reserve downstream of it (e.g., the cadherin-domain protein CLSTN2, or the sphingomyelin synthase, SGMS2). The gene inventory provides a framework to analyze the genetic bases of ovarian development and female fertility. Keywords: reference design Comparison of Foxl2+/+,+/- and -/- whole ovaries at 2 timepoints delineating follicle formation

2010-05-17 | E-GEOD-12942 | biostudies-arrayexpress

Expression data from Foxl2 wild-type and mutant ovaries and testes

Project description:Foxl2 is a forkhead transcription factor expressed only in the female, but not in the male gonad. We have created mice homozygous mutant for the Foxl2 gene (KO) as well as mice carrying a conditional mutant Foxl2 allele (floxed). The expression profiles of conventional Foxl2 knockout and wildtype ovaries were compared at P3, using the Affy Mouse Genome 430 2.0 Array. Adult wildtype and conditional mutant (Foxl2 floxed x RosaCre-EBD treated with tamoxifen) ovaries were compared to adult wildtype testes using the Affymetrix Mouse Gene 1.0 ST Array. Both experiments (KO/WT P3 and Mutant/WT/Testis Adult were also compared to each other.)

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Foxl2 functions in sex determination and histogenesis throughout mouse ovary development, analyzed by Agilent arrays

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Genome wide identification of FOXL2 binding sites in mouse fetal ovaries

Project description:The identity of the gonads is determined by which fate, ovarian granulosa cell or testicular Sertoli cell, the bipotential somatic cell precursors choose to follow. In most vertebrates, the fate of granulosa cells is controlled by a conserved regulator FOXL2. To understand how FOXL2 elicits its fate-determining action, we performed genome-wide analysis of FOXL2 chromatin occupancy in fetal ovaries. Combining genome-wide analysis of FOXL2 binding in the fetal ovary with transcriptomic analyses of Foxl2 gain-of-function and Foxl2 loss-of-function models, we identified potential pathways responsible for the feminizing action of FOXL2. Finally, comparison of FOXL2 genome-wide occupancy in the fetal ovary with testis-determining factor SOX9 genome-wide occupancy in the fetal testis revealed extensive overlaps, implying that antagonistic signals between FOXL2 and SOX9 occur at the chromatin level.

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RUNX1 safeguards the identity of the fetal ovary through an interplay with FOXL2

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ChIP-chip by array of mouse ovaries from wild-type 5 week old Swiss mice using custom anit-Foxl2 polyclonal antibodies

Project description:ChIP-on-Chip experiment using chromatin from wild-type 5 weeks old swiss mice ovaries (Nishi et al, 2001) and an isovolumic blend of our custom anti-Foxl2 polyclonal antibodies (Cocquet J et al, 2002). Non precipitated sheared matched deproteinized chromatin (Input) was used a control to estimate genomic enrichment peaks (and thus Foxl2 binding sites) from IPed DNA. DNA from three independent ChIP assays (Input extractions) was pooled, and 100ng of DNA was linearly amplified using the Whole Genome Amplification kit (Sigma).

2011-02-22 | E-MTAB-400 | biostudies-arrayexpress

Foxl2 functions throughout mouse ovary development

Project description:This SuperSeries is composed of the following subset Series: GSE12905: Foxl2 functions in sex determination and histogenesis throughout mouse ovary development, analyzed by Affymetrix arrays GSE12942: Foxl2 functions in sex determination and histogenesis throughout mouse ovary development, analyzed by Agilent arrays Refer to individual Series

2010-05-17 | E-GEOD-12989 | biostudies-arrayexpress

RUNX1 safeguards the identity of the fetal ovary through an interplay with FOXL2

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2019-04-02 | GSE128767 | GEO

Genome wide identification of FOXL2 binding sites in mouse fetal ovaries

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| PRJNA432737 | ENA