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Transcription profiling of mouse B cells derived from bone marrow

ABSTRACT: Affymetrix GeneChip derived gene expression profiles of flow sorted murine B cell precursors, ex-vivo isolated, RNA-amplified

ORGANISM(S): Mus musculus

SUBMITTER: Reinhard Hoffmann

PROVIDER: E-GEOD-13 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Changes in gene expression profiles in developing B cells of murine bone marrow.

Hoffmann Reinhard R Seidl Thomas T Neeb Martin M Rolink Antonius A Melchers Fritz F

Genome research 20020101 1

Gene expression profiles of five consecutive stages of mouse B cell development were generated with high-density oligonucleotide arrays from as few as 2 x 10(4) ex vivo isolated and flow-cytometrically purified cells. Between 2.8% and 6.8% of all genes change on differentiation from one cellular stage to the next by at least twofold. The entire pathway involves differential expression of 10.7% of all genes. Previously known expression patterns of 15 genes (like surrogate light chain, RAG-1/2, MH ...[more]

PMID: 11779835

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