Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

52 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of the response of Saccharomyces cerevisiae to desiccation and rehydration: Series 2

ABSTRACT: This series represents Experiment 2 of the yeast desiccation / rehydration time course analysis. Samples include Control, 50% dry, Dry, 15 min. post rehydration, 45 min. post rehydration, 90 min. post rehydration, and 360 min. post rehydration.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Richard Helm

PROVIDER: E-GEOD-1312 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Transcriptional response of Saccharomyces cerevisiae to desiccation and rehydration.

Singh Jatinder J Kumar Deept D Ramakrishnan Naren N Singhal Vibha V Jervis Jody J Garst James F JF Slaughter Stephen M SM DeSantis Andrea M AM Potts Malcolm M Helm Richard F RF

Applied and environmental microbiology 20051201 12

A transcriptional analysis of the response of Saccharomyces cerevisiae strain BY4743 to controlled air-drying (desiccation) and subsequent rehydration under minimal glucose conditions was performed. Expression of genes involved in fatty acid oxidation and the glyoxylate cycle was observed to increase during drying and remained in this state during the rehydration phase. When the BY4743 expression profile for the dried sample was compared to that of a commercially prepared dry active yeast, strik ...[more]

PMID: 16332871

Similar Datasets

Transcription profiling of the response of Saccharomyces cerevisiae to desiccation and rehydration: Series 1

2007-10-23 | E-GEOD-1311 | biostudies-arrayexpress

Transcription profiling of the response of Saccharomyces cerevisiae to desiccation and rehydration: Series 3

2007-10-23 | E-GEOD-1313 | biostudies-arrayexpress

Transcription profiling of S. cerevisiae laboratory strain BY4743 and commercial dry active yeast

Project description:This series represents the analysis of a commercial dry active yeast (purchased locally). The genetics of this sample is unknown.

2007-10-23 | E-GEOD-1314 | biostudies-arrayexpress

YDRseries2, Yeast desiccation / rehydration time course

Project description:This series represents Experiment 2 of the yeast desiccation / rehydration time course analysis. Samples include Control, 50% dry, Dry, 15 min. post rehydration, 45 min. post rehydration, 90 min. post rehydration, and 360 min. post rehydration. Keywords = BY4743 Keywords = desiccation Keywords = glucose-limited Keywords = rehydration Keywords = yeast Keywords: time-course

2005-08-01 | GSE1312 | GEO

YDRseries1, Yeast desiccation / rehydration time course

Project description:This series represents Experiment 1 of the yeast desiccation / rehydration time course analysis. Samples include Control, 50% dry, Dry, 15 min. post rehydration, 45 min. post rehydration, 90 min. post rehydration, and 360 min. post rehydration. Keywords = BY4743 Keywords = desiccation Keywords = glucose-limited Keywords = rehydration Keywords = yeast Keywords: time-course

2005-08-01 | GSE1311 | GEO

YDRseries3, Yeast desiccation / rehydration time course

Project description:This series represents Experiment 3 of the yeast desiccation / rehydration time course analysis. Samples include Control, 50% dry, Dry, 15 min. post rehydration, 45 min. post rehydration, 90 min. post rehydration, and 360 min. post rehydration. Keywords = BY4743 Keywords = desiccation Keywords = glucose-limited Keywords = rehydration Keywords = yeast Keywords: time-course

2005-08-01 | GSE1313 | GEO

A Proteomic Analysis of Desiccation Tolerance in Racomitrium canescens

Project description:The moss Racomitrium canescens has strong desiccation tolerance; it can remain almost completely desiccated for years and yet recover within minutes of rehydration. Dissecting the mechanisms underlying such desiccation tolerance in bryophytes could identify candidate proteins that improve plant drought tolerance. The mechanisms involved in recovery from desiccation was explored using label-free-quantitative proteomics comparing desiccated Racomitrium canescens samples rehydrated for 1 min or 6 h.

2023-03-11 | PXD026286 | Pride

Transcription profiling of E.coli W3110 oxygen downshift

Project description:Dynamical response to oxygen downshift under fermentation conditions was tested by taking sample before (S1) and after (S2, S3 and S4) the oxygen downshift. The dynamical changes relevant for ongoing research on physiology were applied. Experiment Overall Design: Four microarray chips were analyzed: Experiment Overall Design: S1 was taken 15 min before the oxygen downshift Experiment Overall Design: S2, S3 and S4 were taken 15 min, 45 min and 75 min after the oxygen downshift, respectively

2008-10-21 | E-GEOD-12006 | biostudies-arrayexpress

The transcriptome response of leaves of the resurrection plant Xerophyta humilis to desiccation

Project description:Measurement of changes in the mRNA transcript abundance of 1709 cDNAs in Xerophyta humilis leaves at 6 relative water contents (RWC) (100%, 80%, 60%, 40%, 20% and 5%RWC respectively). 3105 cDNA clones (corresponding to 1709 unique cDNAs) were randomly selected from X. humilis cDNA libraries (Leaf Dehydration (LD), Leaf Rehydration (LR), Root Dehydration (RD) and Root Rehydration (RR)), amplified by PCR, and printed on each glass slide multiple times (ranging from 4 to 12X), such that each printed block contained a random unbiased mixture of cDNAs from the 4 different cDNA libraries. Total RNA extracted from each X. humilis leaf sample was linearly amplified, labelled with Cy3, and simultaneously hybridized with Cy5 labelled common reference RNA, to the printed cDNA arrays. The reference RNA comprised equal amounts of total RNA pooled from 6 different RWC experimental samples. Data was recorded for five biological replicates for each of the 6 RWC stages being investigated.

2012-03-29 | E-GEOD-34952 | biostudies-arrayexpress

Integrative proteomic and physiological analyses of the molecular response to dessication-stress in Auricularia fibrillifera

Project description:Drought stress is one of the main factors influencing the growth and development of an organism. Auricularia fibrillifera has strong dessication resistance. In A. fibrillifera under dessication-stress, the melanin content of fruiting bodies elevated significantly by >10-fold compared with the control. Folate content also increased sharply but decreased significantly after rehydration, and amino acid and biotin levels increased by 40.11% and 22.14%, respectively. In proteomic analysis, 1,572 and 21 differentially abundant proteins (DAPs) were identified under dessication-stress and rehydration, respectively. A large number of DAPs were annotated in “amino acid metabolism,” “carbohydrate metabolism,” and “translation” pathways, and the DAPs related to osmotic regulation and antioxidant enzymes were significantly increased in abundance. Transcriptome-proteome association analysis showed that most DAPs (30) were annotated in the “biosynthesis of antibiotics” pathway. DAPs and corresponding differentially expressed genes were all up-regulated in the “biotin biosynthesis” pathway and associated with “folate biosynthesis” and “phenylalanine, tyrosine, and tryptophan biosynthesis.” In the analysis of protein–protein interactions, the DAPs annotated in the “phenylalanine, tyrosine, and tryptophan biosynthesis” pathway had the strongest interactions with other DAPs. These enriched pathways could enhance amino acid, folate, biotin, and melanin levels during desiccation stress, which is consistent with the physiological data (amino acid, folate, biotin, and melanin contents). In addition, many DAPs related to the cytoskeleton were significantly increased in abundance under dessication-stress. Physiological and transcriptome data were in agreement with proteomic results. This work provides valuable insight into the dessication-tolerant mechanisms of A. fibrillifera.

2022-09-04 | PXD033449 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data