Mismatch oligonucleotides in human
Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Mark Gerstein
PROVIDER: E-GEOD-13172 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Mismatch oligonucleotides in human and yeast: guidelines for probe design on tiling microarrays.
Seringhaus Michael M Rozowsky Joel J Royce Thomas T Nagalakshmi Ugrappa U Jee Justin J Snyder Michael M Gerstein Mark M
BMC genomics 20081231
<h4>Background</h4>Mismatched oligonucleotides are widely used on microarrays to differentiate specific from nonspecific hybridization. While many experiments rely on such oligos, the hybridization behavior of various degrees of mismatch (MM) structure has not been extensively studied. Here, we present the results of two large-scale microarray experiments on S. cerevisiae and H. sapiens genomic DNA, to explore MM oligonucleotide behavior with real sample mixtures under tiling-array conditions.<h ...[more]
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