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Transcription profiling of mouse wild type and HIF-1a null hepatocyte cultures under short-term hypoxic conditions vs controls

ABSTRACT: The primary aim of this study was to evaluate the changes in hepatocyte gene expression under short-term hypoxic conditions in wild type and HIF-1a null cultures. To this end, hypoxia treated cultures were subjected to incubation with 1% O2/5% CO2/94% N2 at 37Â° C for eight hours prior to RNA isolation. Duplicate normoxic controls were established from separate animals wherein cultures were untreated and treated with Adbgal. Biological triplicates of wild type and HIF-1a null cultures were placed under hypoxic conditions and subsequently processed for microarray analysis. A total of 10 microarray hybridizations were performed.

ORGANISM(S): Mus musculus

SUBMITTER: Jared Allen

PROVIDER: E-GEOD-1321 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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