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Overexpression of Musashi1 (Msi1) in HEK293T cells.

ABSTRACT: Gene expression analysis of the Msi1 overexpression in HEK 293T cells. There were two biological replicates of Msi1 and GFP (Green Fluorescent Protein) as control, used in the experiment. Each slide includes 4 microarrays with one biological sample versus control. Samples were hybridized with dye-swap replica.

ORGANISM(S): Homo sapiens

SUBMITTER: Suzanne Burns

PROVIDER: E-GEOD-13215 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genomic analyses of musashi1 downstream targets show a strong association with cancer-related processes.

de Sousa Abreu Raquel R Sanchez-Diaz Patricia C PC Vogel Christine C Burns Suzanne C SC Ko Daijin D Burton Tarea L TL Vo Dat T DT Chennasamudaram Soudhamini S Le Shu-Yun SY Shapiro Bruce A BA Penalva Luiz O F LO

The Journal of biological chemistry 20090303 18

Musashi1 (Msi1) is a highly conserved RNA-binding protein with pivotal functions in stem cell maintenance, nervous system development, and tumorigenesis. Despite its importance, only three direct mRNA targets have been characterized so far: m-numb, CDKN1A, and c-mos. Msi1 has been shown to affect their translation by binding to short elements located in the 3'-untranslated region. To better understand Msi1 functions, we initially performed an RIP-Chip analysis in HEK293T cells; this method consi ...[more]

PMID: 19258308

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