Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of human peripheral blood cells reveals laughter regulates postprandial blood glucose levels and gene expression

ABSTRACT: Sample tissue: peripheral blood; Disease: diabetes; Samples for gene expression analysis were obtained before the meal and 1.5 hours after the event. Event: listening to a Japanese comic story or a monotonous academic lecture without humor.

ORGANISM(S): Homo sapiens

SUBMITTER: Toshiro Saito

PROVIDER: E-GEOD-1322 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Laughter regulates gene expression in patients with type 2 diabetes.

Hayashi Takashi T Urayama Osamu O Kawai Koichi K Hayashi Keiko K Iwanaga Shizuko S Ohta Masayuki M Saito Toshiro T Murakami Kazuo K

Psychotherapy and psychosomatics 20060101 1

<h4>Background</h4>Positive emotions influence endocrinological and immunological response. This study examined the effect of laughter,as an expression of positive emotion, in terms of gene expression changes.<h4>Methods</h4>Using a microarray technique, we analyzed the changes in expression of 18,716 genes from peripheral blood leukocytes in patients with type 2 diabetes, which were induced by laughter.<h4>Results</h4>Of the 18,716 genes, 23 genes showed significantly different expression chang ...[more]

PMID: 16361876

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Project description:Impaired drainage of aqueous humor through the trabecular meshwork (TM) culminating in increased intraocular pressure is a major risk factor for glaucoma, a leading cause of blindness worldwide. Regulation of aqueous humor drainage through the TM, however, is poorly understood. The role of RhoA GTPase-mediated contractile activity, cell adhesive interactions, and gene expression in regulation of aqueous humor outflow was investigated using adenoviral vector-driven expression of constitutively active RhoA (RhoAV14). Organ cultured anterior segments from porcine eyes expressing RhoAV14 exhibited significant reduction of aqueous humor outflow. Cultured TM cells expressing RhoAV14 revealed strong contractile cell morphology, increased actin stress fibers and focal adhesions, along with increased levels of phosphorylated myosin II, and collagen IV, fibronectin and laminin. cDNA microarray analysis of RNA extracted from RhoAV14 expressing human TM cells revealed a significant increase in the expression of genes encoding extracellular matrix (ECM) proteins, cytokines, integrins, cytoskeletal proteins and signaling proteins. Conversely, various ECM proteins stimulated robust increases in phosphorylation of myosin II, paxillin and focal adhesion kinase, and activated Rho GTPase and actin stress fiber formation in TM cells, indicating a potential regulatory feedback interaction between ECM-induced mechanical strain and Rho GTPase-induced isometric tension in TM cells. Collectively, these data demonstrate that sustained activation of Rho GTPase signaling in the aqueous humor outflow pathway increases resistance to aqueous humor outflow through the trabecular pathway by influencing the contractile force, cell adhesive interactions, and the expression of ECM proteins and cytokines in TM cells. Keywords: Gene Expression Two condition experiment: Human trabecular mesh work cells infected with Adenivirus expressing GFP Vs Adenovirus expressing GFP and constitutively active RhoAV14

Dataset Information

Transcription profiling of human peripheral blood cells reveals laughter regulates postprandial blood glucose levels and gene expression

Publications

Laughter regulates gene expression in patients with type 2 diabetes.

Similar Datasets

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