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Expression analysis to identify inducible genes in T cells


ABSTRACT: Expression microarrays were employed to identify genes induced by phorbol ester and ionomycin stimulation of EL4 cells. EL4 is a murine T cell line. To identify induced genes that were independent of new protein synthesis cells were pre-treated with cycloheximide. This expression study was used in conjunction with histone acetylation ChIP-chip to determine if inducible genes had a specific histone acetylation profile and whether the acetylation profile differed for genes with different kinetics of induction. Keywords: drug treatment comparison Cells were pre-treated with either DMSO or cylcoheximide in DMSO, then they were stimulated with PMA and Ionomycin for either 0h or 4h. 3 biological replicates were used for each treatment-stimulation combination.

ORGANISM(S): Mus musculus

SUBMITTER: Chloe Lim 

PROVIDER: E-GEOD-13278 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Defining the chromatin signature of inducible genes in T cells.

Lim Pek S PS   Hardy Kristine K   Bunting Karen L KL   Ma Lina L   Peng Kaiman K   Chen Xinxin X   Shannon Mary F MF  

Genome biology 20091006 10


<h4>Background</h4>Specific chromatin characteristics, especially the modification status of the core histone proteins, are associated with active and inactive genes. There is growing evidence that genes that respond to environmental or developmental signals may possess distinct chromatin marks. Using a T cell model and both genome-wide and gene-focused approaches, we examined the chromatin characteristics of genes that respond to T cell activation.<h4>Results</h4>To facilitate comparison of gen  ...[more]

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