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Transcription profiling of mouse Rb1 deficient Apc1638N cecal tumors vs duodenal tumors

ABSTRACT: To examine the role of Rb1 in gastrointestinal (GI) tumors we generated mice with an Apc1638N allele, Rbtm2brn floxed alleles, and a villlin-cre transgene (RBVCA). These mice had reduced median survival due to an increase in tumor incidence and multiplicity in the cecum and the proximal colon; they differed from murine intestinal tumors of the Apc1638N type which normally arise solely in the small intestine. We have examined by micro-array analysis three cecal tumors from these mice (probable adenomas), and compared them to three duodenal tumors (probable adenocarcinomas). Expression profiles of duodenal and cecal tumors relative to each other show unique gene subsets up and down regulated. The two tumor types were subsequently shown to differentially regulate distinct sets of genes over expressed in a majority of human colorectal carcinomas. Experiment Overall Design: We have compared 3 cecal tumors with 3 duodenal tumors from Rb1 deficient Apc1638N mice.

ORGANISM(S): Mus musculus

SUBMITTER: Dmitriy Sonkin 

PROVIDER: E-GEOD-13298 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Loss of Rb1 in the gastrointestinal tract of Apc1638N mice promotes tumors of the cecum and proximal colon.

Kucherlapati Melanie H MH   Yang Kan K   Fan Kunhua K   Kuraguchi Mari M   Sonkin Dmitriy D   Rosulek Andrew A   Lipkin Martin M   Bronson Roderick T RT   Aronow Bruce J BJ   Kucherlapati Raju R  

Proceedings of the National Academy of Sciences of the United States of America 20081001 40

To examine the role of Rb1 in gastrointestinal (GI) tumors, we generated mice with an Apc(1638N) allele, Rb(tm2brn) floxed alleles, and a villin-cre transgene (RBVCA). These animals had exon 19 deleted from Rb1 throughout the GI tract. We have shown previously that Rb1 deficiency is insufficient for GI tumor initiation, with inactivation of an Apc allele capable of overcoming the insufficiency. In this study we demonstrate that RBVCA mice have reduced median survival because of an increase in tu  ...[more]

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