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Heterologous Microarray Hybridization Between Vesicomyid Symbionts

ABSTRACT: The goal of this study was to use heterologous microarray hybridization to determine genomic content shared among different vesicomyid symbionts. These symbionts are closely related and can be thought of as different strains of bacteria, facilitating the use of heterologous microarray hybridization to determine genomic content. Keywords: comparative genomic hybridization Microarrays were built off the Ruthia magnifica genome and two replicate hybridizations to this organism were used as a baseline for comparisons. Genomic DNA from two other vesicomyid symbionts (Calyptogena kilmeri and C. pacifica symbionts) was also hybridized to the array with three biological replicates for each sample.

ORGANISM(S): Calyptogena magnifica symbiont

SUBMITTER: Irene Newton

PROVIDER: E-GEOD-13447 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Comparative genomics of vesicomyid clam (Bivalvia: Mollusca) chemosynthetic symbionts.

Newton Irene L G IL Girguis Peter R PR Cavanaugh Colleen M CM

BMC genomics 20081204

<h4>Background</h4>The Vesicomyidae (Bivalvia: Mollusca) are a family of clams that form symbioses with chemosynthetic gamma-proteobacteria. They exist in environments such as hydrothermal vents and cold seeps and have a reduced gut and feeding groove, indicating a large dependence on their endosymbionts for nutrition. Recently, two vesicomyid symbiont genomes were sequenced, illuminating the possible nutritional contributions of the symbiont to the host and making genome-wide evolutionary analy ...[more]

PMID: 19055818

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Heterologous Microarray Hybridization Between Vesicomyid Symbionts

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