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Transcription profiling time series of human bone marrow samples stored at room temperature for up to 48 hours after aspiration

ABSTRACT: Impact of pre-analytical handling on Bone Marrow (BM) mRNA Gene Expression: We have investigated the impact of RNA extraction protocols and time delays (24 and 48h) between sample aspiration and RNA extraction on RNA quality and gene expression profiles. Intact RNA can be extracted from BM samples stored at room temperature for up to 48h after aspiration. However, storage of BM has dramatic effects on mRNA expression of individual transcripts.

ORGANISM(S): Homo sapiens

SUBMITTER: Matthias Nees

PROVIDER: E-GEOD-1347 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Impact of pre-analytical handling on bone marrow mRNA gene expression.

Breit Stephen S Nees Matthias M Schaefer Ulrike U Pfoersich Margit M Hagemeier Christian C Muckenthaler Martina M Kulozik Andreas E AE

British journal of haematology 20040701 2

Large clinical trials on leukaemia, require the transport of bone marrow (BM) from participating clinics to central diagnostic laboratories. We have investigated the impact of RNA extraction protocols and time delays between sample aspiration and RNA extraction on RNA quality and gene expression profiles. Intact RNA can be extracted from BM samples stored at room temperature for up to 48 h. Gene expression analyses using Affymetrix U95Av2 GeneChips and a custom-designed cDNA array in parallel sh ...[more]

PMID: 15238145

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Pre-analytical changes of mRNA Gene Expression as the result from prolonged storage

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