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Profiling of Transcripts in Rat Proximal Tubule

ABSTRACT: Freshly isolated rat kidney proximal tubules were subjected for transcript profiling. Three microarray experiments were done to obtain the kidney proxmial tubule transcriptome.

ORGANISM(S): Rattus norvegicus

SUBMITTER: Ming-Jiun Yu

PROVIDER: E-GEOD-13668 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Systems-level analysis of cell-specific AQP2 gene expression in renal collecting duct.

Yu Ming-Jiun MJ Miller R Lance RL Uawithya Panapat P Rinschen Markus M MM Khositseth Sookkasem S Braucht Drew W W DW Chou Chung-Lin CL Pisitkun Trairak T Nelson Raoul D RD Knepper Mark A MA

Proceedings of the National Academy of Sciences of the United States of America 20090203 7

We used a systems biology-based approach to investigate the basis of cell-specific expression of the water channel aquaporin-2 (AQP2) in the renal collecting duct. Computational analysis of the 5'-flanking region of the AQP2 gene (Genomatix) revealed 2 conserved clusters of putative transcriptional regulator (TR) binding elements (BEs) centered at -513 bp (corresponding to the SF1, NFAT, and FKHD TR families) and -224 bp (corresponding to the AP2, SRF, CREB, GATA, and HOX TR families). Three oth ...[more]

PMID: 19190182

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