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Transcription profiling of human mononuclear cells from juvenile arthritis and spondyloarthropathy patients

ABSTRACT: Biotinylated cRNA was synthesized from total RNA (Enzo; Farmingdale, NY) and processed according to the Affymetrix GeneChip Expression Analysis Technical Manual (Affymetrix; Santa Clara, CA). 57 samples: 5 pauciarticular PBMC, 15 polyarticular PBMC, 11 control PMBC, 6 JSpA PBMC, 5 pauciarticular SFMC, 10 polyarticular PBMC, 5 JSpA SFMC classified by course.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael Barnes

PROVIDER: E-GEOD-1402 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression in juvenile arthritis and spondyloarthropathy: pro-angiogenic ELR+ chemokine genes relate to course of arthritis.

Barnes M G MG Aronow B J BJ Luyrink L K LK Moroldo M B MB Pavlidis P P Passo M H MH Grom A A AA Hirsch R R Giannini E H EH Colbert R A RA Glass D N DN Thompson S D SD

Rheumatology (Oxford, England) 20040518 8

<h4>Objective</h4>To evaluate the ability of microarray-based methods to identify genes with disease-specific expression patterns in peripheral blood mononuclear cells (PBMC) and synovial fluid mononuclear cells (SFMC) of juvenile arthritis patients and healthy controls.<h4>Methods</h4>Microarray data (Affymetrix U95Av2) from 26 PBMC and 20 SFMC samples collected from patients with active disease (classified by course according to ACR criteria) were analysed for expression patterns that correlat ...[more]

PMID: 15150433

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