Dataset Information
Exploration of essential gene functions via titratable promoter alleles
Ontology highlight
ABSTRACT:
ORGANISM(S): Saccharomyces cerevisiae
SUBMITTER: Timothy Hughes
PROVIDER: E-GEOD-1404 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Publications
Exploration of essential gene functions via titratable promoter alleles.
Cell 20040701 1
Nearly 20% of yeast genes are required for viability, hindering genetic analysis with knockouts. We created promoter-shutoff strains for over two-thirds of all essential yeast genes and subjected them to morphological analysis, size profiling, drug sensitivity screening, and microarray expression profiling. We then used this compendium of data to ask which phenotypic features characterized different functional classes and used these to infer potential functions for uncharacterized genes. We iden ...[more]
