Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human SupT1, PBMC or IMR90 cells infected with an HIV-based vector

ABSTRACT: SupT1, PBMC, or IMR90 cells were infected with an HIV-based vector (see Schroder et al., Cell 110:521-9) and the RNA isolated 48 hours after infection. ***PLEASE NOTE***; Series entries GSE1408 & GSE1409 no longer exist. All data discussed in the author's PLoS publication (PubMed ID 15314653) is available under GSE1407 & GSE1410. ***PLEASE NOTE***

ORGANISM(S): Homo sapiens

SUBMITTER: Richard Steven Mitchell 

PROVIDER: E-GEOD-1407 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Retroviral DNA integration: ASLV, HIV, and MLV show distinct target site preferences.

Mitchell Rick S RS   Beitzel Brett F BF   Schroder Astrid R W AR   Shinn Paul P   Chen Huaming H   Berry Charles C CC   Ecker Joseph R JR   Bushman Frederic D FD  

PLoS biology 20040817 8

The completion of the human genome sequence has made possible genome-wide studies of retroviral DNA integration. Here we report an analysis of 3,127 integration site sequences from human cells. We compared retroviral vectors derived from human immunodeficiency virus (HIV), avian sarcoma-leukosis virus (ASLV), and murine leukemia virus (MLV). Effects of gene activity on integration targeting were assessed by transcriptional profiling of infected cells. Integration by HIV vectors, analyzed in two  ...[more]

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