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Transcription profiling of human synchronized HTC116 cells treated with DMSO or Nocodazole time course

ABSTRACT: Analysis of synchronized HCT116 cells at various time points up to 10 hours following treatment with DMSO or Nocodazole. Experiment Overall Design: HCT116 colorectal cancer cell line was obtained from the American Type Culture Collection (ATCC) and grown in McCOYâS 5A MEDIUM MODIFIED (SIGMA) with 10% FBS (JBS). It was maintained at 37Â°C and 5% CO2. Synchronous culture was obtained by incubating cells for 19 h in 2 mM of thymidine, followed by a 9 h-incubation in normal medium and a second 16 h-incubation in thymidine (2 mM). Cells were washed with normal medium followed by treatment with DMSO for 0, 2, 4, 6, 7, 8, 9 and 10 h as a control or 0.1 mg/ml Nocodazole (Sigma) for 7, 8, 9 and 10 h.

ORGANISM(S): Homo sapiens

SUBMITTER: Hideaki Mizuno

PROVIDER: E-GEOD-14103 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A signature-based method for indexing cell cycle phase distribution from microarray profiles.

Mizuno Hideaki H Nakanishi Yoshito Y Ishii Nobuya N Sarai Akinori A Kitada Kunio K

BMC genomics 20090330

<h4>Background</h4>The cell cycle machinery interprets oncogenic signals and reflects the biology of cancers. To date, various methods for cell cycle phase estimation such as mitotic index, S phase fraction, and immunohistochemistry have provided valuable information on cancers (e.g. proliferation rate). However, those methods rely on one or few measurements and the scope of the information is limited. There is a need for more systematic cell cycle analysis methods.<h4>Results</h4>We developed a ...[more]

PMID: 19331659

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