Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Gene Expression Profiling of the MLL-AF4 and Flt3 tyrosine kinase domain (TKD) genes in 32Dc cell


ABSTRACT: The tumorigenesis capacity of MLL-AF4 alone is insufficient for causing leukemia. Based on the finding that an Flt3 gene mutation in the tyrosine kinase domain (TKD) was observed in approximately 15% of MLL leukemia, we investigated synergistic leukemogenesis effects of the two genes in vitro. In a mouse IL3-dependent cell line, 32Dc, the expression of MLL-AF4 and Flt3 TKD was induced using a lentiviral vector. We performed gene expression profiling in the MLL-AF4 and the Flt3 TKD+MLL-AF4 expressing 32Dc cells. The enhancement of Hox genes expression was not identified. However, instead, the expression of S100A6, which was involved in the control of cell proliferation, was synergistically enhanced in the presence of both MLL-AF4 and Flt3 TKD genes. We performed gene expression profiling: 32Dc vs. MLL-AF4 expressing 32Dc, 32Dc vs. Flt3 TKD+MLL-AF4 expressing 32Dc, and MLL-AF4 expressing 32Dc vs. Flt3 TKD+MLL-AF4 expressing 32Dc. A single sample for each expressing cells was analyzed.

ORGANISM(S): Mus musculus

SUBMITTER: Hiroki Yamaguchi 

PROVIDER: E-GEOD-14236 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Multistep pathogenesis of leukemia via the MLL-AF4 chimeric gene/Flt3 gene tyrosine kinase domain (TKD) mutation-related enhancement of S100A6 expression.

Yamaguchi Hiroki H   Hanawa Hideki H   Uchida Naoya N   Inamai Mitsuharu M   Sawaguchi Kazuhiro K   Mitamura Yoshio Y   Shimada Takashi T   Dan Kazuo K   Inokuchi Koiti K  

Experimental hematology 20090601 6


<h4>Objective</h4>Concerning MLL-AF4 leukemogenesis, previous mouse models suggest that the tumorigenesis capacity of MLL-AF4 alone is insufficient for causing leukemia. Based on the finding that an Fms-like tyrosine kinase 3 (Flt3) gene mutation in the tyrosine kinase domain (TKD) was observed in approximately 15% of mixed lineage leukemia (MLL), we investigated synergistic leukemogenesis effects of the two genes in vitro.<h4>Materials and methods</h4>In a mouse interleukin-3 (IL-3)-dependent c  ...[more]

Similar Datasets

2009-03-31 | GSE14236 | GEO
2013-06-04 | E-GEOD-40103 | biostudies-arrayexpress
2013-06-04 | GSE40103 | GEO
2011-06-09 | E-GEOD-29869 | biostudies-arrayexpress
2021-08-11 | GSE156115 | GEO
2023-08-18 | PXD043920 | Pride
2016-11-23 | GSE85988 | GEO
2018-07-21 | GSE71140 | GEO
2021-10-25 | GSE162032 | GEO
2011-06-09 | GSE29869 | GEO