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A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress

ABSTRACT: We identify inducible binding of the transcription factor Oct1 to numerous targets following exposure to hydrogen peroxide. Oct1 bound ChIP fragments were sequenced in the presence and absence of H2O2 exposure using HeLa cells. Both 26 and 36bp reads were generated. Each processed file contains a list of genomic regions (NCBI 36.1, H_sapiens_Mar_2006, hg18) enriched for Oct1 binding. These files were generated by running the EnrichedRegionMaker on ScanSeqs window scored data, see http://useq.sourceforge.net/ . Adjacent and overlapping windows with a q-value FDR of 0.0001 were joined into larger enriched regions. The best scoring window's scores are used to represent the enriched region.

ORGANISM(S): Homo sapiens

SUBMITTER: Dean Tantin

PROVIDER: E-GEOD-14283 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A general mechanism for transcription regulation by Oct1 and Oct4 in response to genotoxic and oxidative stress.

Kang Jinsuk J Gemberling Matthew M Nakamura Mitsuhiro M Whitby Frank G FG Handa Hiroshi H Fairbrother William G WG Tantin Dean D

Genes & development 20090101 2

Oct1 and Oct4 are homologous transcription factors with similar DNA-binding specificities. Here we show that Oct1 is dynamically phosphorylated in vivo following exposure of cells to oxidative and genotoxic stress. We further show that stress regulates the selectivity of both proteins for specific DNA sequences. Mutation of conserved phosphorylation target DNA-binding domain residues in Oct1, and Oct4 confirms their role in regulating binding selectivity. Using chromatin immunoprecipitation, we ...[more]

PMID: 19171782

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