Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Effects of DEHP on the hepatic expression of a selection of mouse genes related to nuclear receptor signaling.


ABSTRACT: Phthalates are industrial additives widely used as plasticizers. In addition to deleterious effects on male genital development, population studies have documented correlations between phthalates exposure and impacts on reproductive tract development and on the metabolic syndrome in male adults. In this study we investigated potential mechanisms underlying the impact of di-(2-ethylhexyl)-phthalate (DEHP) on adult mouse liver in vivo. A parallel analysis of hepatic transcript and metabolic profiles from adult mice exposed to varying DEHP doses was performed. Hepatic genes modulated by DEHP are predominantly PPARα targets. However, the induction of prototypic cytochrome P450 genes strongly supports the activation of additional NR pathways, including Constitutive Androstane Receptor (CAR). Integration of transcriptomic and metabonomic profiles revealed a correlation between the impacts of DEHP on genes and metabolites related to heme synthesis and on the Rev-erbα pathway that senses endogenous heme level. Keywords: Treatment effect One-color macroarrays, 4 experimental conditions: Control mice (vehicle treated), mice treated with di-(2-ethylhexyl)-phthalate (DEHP) at 30 mg/kg/day (D30), 180 mg/kg/day (D180) or 1100 mg/kg/day (D1100) for 14 days, Biological replicates: 6 controls, 4 D30, 4 D180, 5 D1100, One replicate per array

ORGANISM(S): Mus musculus

SUBMITTER: Pascal GP Martin 

PROVIDER: E-GEOD-14629 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

altmetric image

Publications

Identification of potential mechanisms of toxicity after di-(2-ethylhexyl)-phthalate (DEHP) adult exposure in the liver using a systems biology approach.

Eveillard Alexandre A   Lasserre Frédéric F   de Tayrac Marie M   Polizzi Arnaud A   Claus Sandrine S   Canlet Cécile C   Mselli-Lakhal Laïla L   Gotardi Gaëlle G   Paris Alain A   Guillou Hervé H   Martin Pascal G P PG   Pineau Thierry T  

Toxicology and applied pharmacology 20090223 3


Phthalates are industrial additives widely used as plasticizers. In addition to deleterious effects on male genital development, population studies have documented correlations between phthalates exposure and impacts on reproductive tract development and on the metabolic syndrome in male adults. In this work we investigated potential mechanisms underlying the impact of DEHP on adult mouse liver in vivo. A parallel analysis of hepatic transcript and metabolic profiles from adult mice exposed to v  ...[more]

Similar Datasets

2010-05-22 | E-GEOD-14920 | biostudies-arrayexpress
2009-04-10 | E-GEOD-13237 | biostudies-arrayexpress
2009-04-10 | E-GEOD-13240 | biostudies-arrayexpress
2024-06-17 | E-MTAB-13716 | biostudies-arrayexpress
2024-07-18 | PXD047061 | Pride
2012-10-30 | E-GEOD-41901 | biostudies-arrayexpress
2009-02-19 | GSE14629 | GEO
2009-03-07 | E-GEOD-15016 | biostudies-arrayexpress
2009-02-26 | GSE14920 | GEO
2023-11-30 | GSE246030 | GEO