Temporal regulatory role of miR156 during leaf ontogenesis
Ontology highlight
ABSTRACT: Analysis of different leaves from transgenic plants over expressing microRNA miR156. Time-dependent gene expression is essential for developmental timing control. MiR156 was characterized as a time-dependent expressed microRNA during leaf development. The level of miR156 is positive correlated to developmental time during leaf growth. We used microarray to identify genes regulated by miR156 in a time-dependent manner during leaf development. Results indicate that most time-negative genes were activated in mature leaves of miR156 over expressed plants compared with wild type. Keywords: gene expression array-based (RNA / in situ oligonucleotide) To identify the temporal regulation role of miR156 in different leaves, all leaves in the main tiller were collected separately from Md/Mh (miR156 over expressed lines) and wild type (WT) plants. The level of miR156 were checked in these samples, then three biological repeats were selected and these RNA samples of the oldest (L1) and youngest leaf (L4 or L5) were applied for microarray hybridization.
ORGANISM(S): Oryza sativa Japonica Group
SUBMITTER: Lizhong Xiong
PROVIDER: E-GEOD-14692 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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