Transcription profiling of mouse bone marrow-derived macrophages simulated with LPS, time series

ABSTRACT: The innate immune system is a two-edged sword; it is absolutely required for host defense against infection, but if left uncontrolled can trigger a plethora of inflammatory diseases. Here we used systems biology approaches to predict and validate a gene regulatory network involving a dynamic interplay between the transcription factors NF-κB, C/EBPδ, and ATF3 that controls inflammatory responses. We mathematically modeled transcriptional regulation of Il6 and Cebpd genes and experimentally validated the prediction that the combination of an initiator (NF-κB), an amplifier (C/EBPδ) and an attenuator (ATF3) forms a regulatory circuit that discriminates between transient and persistent Toll-like receptor 4-induced signals. Our results suggest a mechanism that enables the innate immune sy

ORGANISM(S): Mus musculus

SUBMITTER: Bruz Marzolf 

PROVIDER: E-GEOD-14769 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress