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Investigation of over-expressing Annexin receptor cell line with and without agonists

ABSTRACT: The therapeutic potential of pro-resolution factors in determining the outcome of inflammatory events has gained ground over the past decade. However, the attention has been focused on the non-genomic effects of these endogenous, anti-inflammatory substances. In this study, we have focused our attention on identifying specific annexin 1 (AnxA1) protein/ALX receptor mediated gene activation, in an effort to identify down-stream genomic targets of this well-known, glucocorticoid induced, pro-resolution factor. In this study, we used four different types of samples (with 2 replicates each):1)cells transfected with the empty pGEX-4T-327 vector and vehicle (PBS) treated; 2)cells transfected with the annexin receptor ALXR (also known as FPR2) and vehicle (PBS) treated; 3)cells transfected with the annexin receptor ALXR and treated with 10uM Ac-26 peptide for 4 hours; 4) cells transfected with the annexin receptor ALXR and treated with 0.5uM Annexin 1 for 4 hours. After the treatments, cells were collected for RNA extraction.

ORGANISM(S): Homo sapiens

SUBMITTER: Trinidad Montero 

PROVIDER: E-GEOD-14807 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Downstream gene activation of the receptor ALX by the agonist annexin A1.

Renshaw Derek D   Montero-Melendez Trinidad T   Dalli Jesmond J   Kamal Ahmad A   Brancaleone Vincenzo V   D'Acquisto Fulvio F   Cirino Giuseppe G   Perretti Mauro M  

PloS one 20100917 9

<h4>Background</h4>Our understanding of pro-resolution factors in determining the outcome of inflammation has recently gained ground, yet not many studies have investigated whether specific genes or patterns of genes, are modified by these mediators. Here, we have focussed on the glucocorticoid modulated pro-resolution factor annexin A1 (AnxA1), studying if its interaction with the ALX receptor would affect downstream genomic targets.<h4>Methodology/principal findings</h4>Using microarray techno  ...[more]

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