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Transcriptional profiling of MLL5 Knock down vs. control C2C12 myoblasts (S phase enriched)

ABSTRACT: Knockdown of MLL5 led to deregulation of S phase. To understand the molecular basis for this phenotype, we performed microarray analysis of S phase synchronized myoblasts. Genes differentially regulated by MLL5 knock down were revealed by microarray analysis using NIA15K mouse chips. Control and knock down cells were synchronized at G0 by suspension culture and reactivated to enter S phase by replating for 24hrs in growth medium.

ORGANISM(S): Mus musculus

SUBMITTER: Prashanth Kandalla

PROVIDER: E-GEOD-14931 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation.

Sebastian Soji S Sreenivas Prethish P Sambasivan Ramkumar R Cheedipudi Sirisha S Kandalla Prashanth P Pavlath Grace K GK Dhawan Jyotsna J

Proceedings of the National Academy of Sciences of the United States of America 20090305 12

Most cells in adult tissues are nondividing. In skeletal muscle, differentiated myofibers have exited the cell cycle permanently, whereas satellite stem cells withdraw transiently, returning to active proliferation to repair damaged myofibers. We have examined the epigenetic mechanisms operating in conditional quiescence by analyzing the function of a predicted chromatin regulator mixed lineage leukemia 5 (MLL5) in a culture model of reversible arrest. MLL5 is induced in quiescent myoblasts and ...[more]

PMID: 19264965

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