Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

47 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profiling of MLL5 Knock down vs. control C2C12 myoblasts (S phase enriched)

ABSTRACT: Knockdown of MLL5 led to deregulation of S phase. To understand the molecular basis for this phenotype, we performed microarray analysis of S phase synchronized myoblasts. Genes differentially regulated by MLL5 knock down were revealed by microarray analysis using NIA15K mouse chips. Control and knock down cells were synchronized at G0 by suspension culture and reactivated to enter S phase by replating for 24hrs in growth medium.

ORGANISM(S): Mus musculus

SUBMITTER: Prashanth Kandalla

PROVIDER: E-GEOD-14931 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

MLL5, a trithorax homolog, indirectly regulates H3K4 methylation, represses cyclin A2 expression, and promotes myogenic differentiation.

Sebastian Soji S Sreenivas Prethish P Sambasivan Ramkumar R Cheedipudi Sirisha S Kandalla Prashanth P Pavlath Grace K GK Dhawan Jyotsna J

Proceedings of the National Academy of Sciences of the United States of America 20090305 12

Most cells in adult tissues are nondividing. In skeletal muscle, differentiated myofibers have exited the cell cycle permanently, whereas satellite stem cells withdraw transiently, returning to active proliferation to repair damaged myofibers. We have examined the epigenetic mechanisms operating in conditional quiescence by analyzing the function of a predicted chromatin regulator mixed lineage leukemia 5 (MLL5) in a culture model of reversible arrest. MLL5 is induced in quiescent myoblasts and ...[more]

PMID: 19264965

Similar Datasets

Distinct transcriptional networks in quiescent myoblasts: a role for Wnt signaling in reversible vs irreversible arrest

Project description:Using cDNA arrays, we compared G0 myoblasts (S48) with post-mitotic myotubes. Our findings define the transcriptional program of quiescent myoblasts in culture which show important similarities with muscle satellite cells, and establish that distinct gene expression profiles characterize irreversible and reversible arrest Cell were synchronized in G0 by resuspending them in semi-solid media for 48 hours and harvested for RNA isolation

2013-04-16 | E-GEOD-33676 | biostudies-arrayexpress

Transcriptional profiling of MLL5 Knock down vs. control C2C12 myoblasts (S phase enriched)

Project description:Knockdown of MLL5 led to deregulation of S phase. To understand the molecular basis for this phenotype, we performed microarray analysis of S phase synchronized myoblasts. Genes differentially regulated by MLL5 knock down were revealed by microarray analysis using NIA15K mouse chips.

2009-03-04 | GSE14931 | GEO

Transcriptional profiling of PRDM2 Knock down vs.controlGFPsh proliferating C2C12 Myoblasts

Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of growing myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using NIA15K mouse chips. Control and knock down cells were grown in proliferating conditions

2015-06-30 | E-GEOD-58675 | biostudies-arrayexpress

Gene expression profiling of PRDM2 Knockdown in proliferating, quiescent and differentiating myoblasts

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2015-06-30 | E-GEOD-58676 | biostudies-arrayexpress

Murine Histone Methyltransferase Mll5, is involved in Male Gametogenesis

Project description:Male mice but not female Mll5 -/- mice are infertile. This study showed that post-meiotic spermatogenic maturation is impaired in Mll5 -/- mice. In order to investigate the role of Mll5 in spermatogenesis, a transcriptome analysis of whole testes from Mll5 knock out and wildtype mice was performed. Affymetrix Mouse Exon chips were hybridized with testes material from three individual male mice from each wild-type or Mll5 -/- genotype, in order to identify gene regulation differences attributed to the loss of Mll5.

2010-09-30 | GSE19648 | GEO

Murine Histone Methyltransferase Mll5, is involved in Male Gametogenesis

2010-09-30 | E-GEOD-19648 | biostudies-arrayexpress

Mus musculus

Project description:Transcriptional profiling of MLL5 Knock down vs. control C2C12 myoblasts (S phase enriched)

| PRJNA111923 | ENA

Quantitative proteome profiling of Caenorhabditis elegans pept-1(lg601) and N2(Bristol) during ontogenesis.

Project description:PEPT-1 is responsible for the uptake of di-and tripeptides form the intestinal lumen into the epithelial cells. Knock-out of pept-1 in C.elegans results in a severe phenotype. Despite a reduction of brood size and a retarded development, pept-1(lg601) depicts increased stress resistance. Therefore, populations of pept1(lg601) as well as N2(Bristol) were synchronized and sampled at various time points during development. Applying 15N metabolic labeling in combination with shotgun LC/MS-MS, we determined the proteome of both strains during development.

2016-07-15 | PXD000310 | Pride

Transcriptional profiling of PRDM2 Knock down vs.controlGFPsh Quiescent C2C12 Myoblasts (G0 enriched)

Project description:Knockdown of PRDM2 led to precocious differentiation. To understand the molecular basis for this phenotype, we performed microaary analysis of quiescent myoblasts. Genes differentially regulated by PRDM2 knock down were reveraled by microarray analysis using affymetrix mouse chips. Control and knock down cells were synchronized at G0 by suspension culture and total RNA was used to perform microarray analysis

2015-06-30 | E-GEOD-58674 | biostudies-arrayexpress

The NuA4 complex promotes Translesion Synthesis (TLS) - mediated DNA damage tolerance

Project description:DNA lesions can block a replication fork, leading to its collapse and gross chromosomal rearrangements. To circumvent such outcomes, DNA damage tolerance (DDT) pathways become engaged, allowing the replisome to bypass the lesion and complete S phase in the presence of unrepaired damage. Here we demonstrate a newly identified role for NuA4, including complex components Esa1 and Yng2, on the Translesion Synthesis (TLS) branch of DDT. Moreover, Our data suggest that NuA4 functionality within the tolerance pathway is likely direct as genome-wide transcriptional analysis with esa1-L254P mutants showed little changes in the expression of TLS factors compared to wild type during MMS treatment. When Yng2 expression is restricted to G2/M, cell viability and mutagenesis rates are restored to the levels measured when only the error-free branch of DDT is disrupted, indicating that the critical role of NuA4 in TLS functions in G2, after chromosomal replication is complete. Lastly, disruption of HTZ1, the Saccharomyces cerevisiae histone variant H2A.Z and target of NuA4, exhibits mutagenic rates of reversion that are comparable to the levels measured with NuA4 complex mutants, esa1-L254P and yng2M-NM-^T. The esa1-L254P strain was compared to wild type through a series of microarrays utilizing dye swaps with and without treatment of MMS. These included synchronized cells in G1 and S phase through alpha factor treatment. Four unique microarrays were performed each with dye swaps, comparing wild type to esa1-L254P in G1 and S phase with and without treatment with MMS. As a set of controls, four microarrays were performed comparing identical strains with and without MMS in G1 or S phase.

2015-02-21 | E-GEOD-66176 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data