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Transcription profiling of Drosophila virgin mated to normal or spermless males to identify denes regulated by mating, sperm or Acps

ABSTRACT: Comparison of gene expression between females that were either virgin, mated to normal males, mated to spermless males (sons of tudor) or mated to sperm-less/Acp-less males (DTA-E).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Lisa McGraw

PROVIDER: E-GEOD-1507 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genes regulated by mating, sperm, or seminal proteins in mated female Drosophila melanogaster.

McGraw Lisa A LA Gibson Greg G Clark Andrew G AG Wolfner Mariana F MF

Current biology : CB 20040801 16

In Drosophila melanogaster, sperm and accessory gland proteins ("Acps," a major component of seminal fluid) transferred by males during mating trigger many physiological and behavioral changes in females (reviewed in ). Determining the genetic changes triggered in females by male-derived molecules and cells is a crucial first step in understanding female responses to mating and the female's role in postcopulatory processes such as sperm competition, cryptic female choice, and sexually antagonist ...[more]

PMID: 15324670

Similar Datasets

Genes regulated by mating, sperm or Acps

Project description:Comparison of gene expression between females that were either virgin, mated to normal males, mated to spermless males (sons of tudor) or mated to sperm-less/Acp-less males (DTA-E). Keywords: other

2004-12-09 | GSE1507 | GEO

Ceratitis capitata gene expression related to mating status in mature males (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males

2011-12-25 | GSE19608 | GEO

Ceratitis capitata gene expression related to mating status in mature males (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata, males. Samples where made from the heads of the males.

2011-12-25 | E-GEOD-19608 | biostudies-arrayexpress

Ceratitis capitata gene expression related to mating status in mature females (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females

2011-12-25 | GSE19573 | GEO

Regulation of Anopheles gambiae male accessory gland genes influences post-mating response in female

Project description:In Drosophila, the accessory gland proteins (Acps) secreted from the male accessory glands (MAGs) and transferred along with sperm into the female reproductive tract have been implicated in triggering postmating behavioral changes, including refractoriness to subsequent mating and propensity to egg laying. Recently, Acps have been found also in Anopheles, suggesting similar functions. Understanding the mechanisms underlying transcriptional regulation of Acps and their functional role in modulating Anopheles postmating behavior may lead to the identification of novel vector control strategies to reduce mosquito populations. We identified heat-shock factor (HSF) binding sites within the Acp promoters of male Anopheles gambiae and discovered three distinct Hsf isoforms; one being significantly up-regulated in the MAGs after mating. Through genome-wide transcription analysis of Hsf-silenced males, we observed significant down-regulation in 50% of the Acp genes if compared to control males treated with a construct directed against an unrelated bacterial sequence. Treated males retained normal life span and reproductive behavior compared to control males. However, mated wild-type females showed a ∼46% reduction of egg deposition rate and a ∼23% reduction of hatching rate (∼58% combined reduction of progeny). Our results highlight an unsuspected role of HSF in regulating Acp transcription in A. gambiae and provide evidence that Acp down-regulation in males leads a significant reduction of progeny, thus opening new avenues toward the development of novel vector control strategies.

2013-02-08 | GSE27233 | GEO

Ceratitis capitata gene expression related to mating status in mature females (mated vs virgin)

Project description:This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females This analysis compares gene expression between 4 day old mated and 4 day old virgin medfly, Ceratitis capitata females. Samples where made from the heads of the females.

2011-12-25 | E-GEOD-19573 | biostudies-arrayexpress

Transcriptome analysis of virgin and mated Anopheles gambiae and Anopheles albimanus

Project description:We custom-built a bioinformatics pipeline to search for 20E-modifying enzymes in the accessory glands of Anopheles gambiae males, searching for ecdysteroid kinases (EcK), ecdysone oxidases (EO), and ecdysteroid-phosphate phosphatases (EPP). To this end, we generated RNAseq datasets of different An. gambiae tissues dissected from virgin and mated females and males, and produced similar datasets for Anopheles albimanus, a South American species that does not synthetize and transfer ecdysteroids during mating. These analyses led to the identification of one candidate EPP and two potential EcKs (EcK1 and EcK2), which we demonstrated are involved in the activity of a male-specific oxidized ecdysteroid (3D20E). We further determined that 3D20E is specifically produced by the An. gambiae male accessory glands and is transferred to females during copulation, where it triggers a series of post-mating responses.

2022-04-20 | GSE198665 | GEO

Mouse utero-tubular junction (UTJ): 1: females in estrous no mating; 2: females in estrous mated with vasectomized male; 3: females in estrous mated with normal male: 1 vs. 2; 1 vs. 3; 2 vs. 3.

Project description:Gene expression profiles comparing controls (1) with females mated with vasectomized male (2) or normal males (3).

2014-04-10 | GSE56620 | GEO

Planococcus citri mealybug virgin and mated female transcriptome sequencing

Project description:P. citri mealybugs cause severe damage in citrus production. We aimed at indentifying genes involved in mealybug sex pheromone biosynthesis. As these should be expressed in virgin females and are presumably downregulated after mating, we looked at genes differentially expressed between mated and virgin females.

2024-02-15 | GSE179660 | GEO

Fire ant alate virgin and de-alate mated queen brain transcriptomes

Project description:Transcriptomes of dissected brains from alate virgin and de-alate mated queens from polygyne fire ants were analyzed and compared. Four replicates of each condition were obtained. Thirteen genes were upregulated in mated queen brain and nine were downregulated. We found that for four differentially expressed genes in brain selected for qPCR analyses, changes in gene expression were most likely driven by the changes in physiological state (i.e. age, nutritional status or dominance rank) or in social environment (released from influence of primer pheromone).

2018-05-09 | GSE108063 | GEO

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