Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Transcription profiling of Arabidopsis seed compartments at the mature green stage.

ABSTRACT: We collected mature green seed compartments from 7 micron paraffin sections using the Leica LMD6000 system in order to identify the mRNAs present in different compartments from seeds containing mature green-stage embryos. For the purposes of this study we captured 6 compartments: embryo proper, micropylar endosperm, cellularized peripherial endosperm, chalazal endosperm, chalazal seed coat and seed coat, as well sets of serial sections encompassing the entire mature green stage seed. Experiment Overall Design: Mature green stage seed compartments were isolated using the LMD6000 system. Total RNA was amplified and hybridized with Affymetrix ATH1 Arabidopsis array for 14 samples (embryo proper, micropylar endosperm, peripherial endosperm, chalazal endosperm, chalazal seed coat, seed coat and whole seeds, 2 biological replicates each).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Ryan Christopher Kirkbride 

PROVIDER: E-GEOD-15165 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Global analysis of gene activity during Arabidopsis seed development and identification of seed-specific transcription factors.

Le Brandon H BH   Cheng Chen C   Bui Anhthu Q AQ   Wagmaister Javier A JA   Henry Kelli F KF   Pelletier Julie J   Kwong Linda L   Belmonte Mark M   Kirkbride Ryan R   Horvath Steve S   Drews Gary N GN   Fischer Robert L RL   Okamuro Jack K JK   Harada John J JJ   Goldberg Robert B RB  

Proceedings of the National Academy of Sciences of the United States of America 20100412 18

Most of the transcription factors (TFs) responsible for controlling seed development are not yet known. To identify TF genes expressed at specific stages of seed development, including those unique to seeds, we used Affymetrix GeneChips to profile Arabidopsis genes active in seeds from fertilization through maturation and at other times of the plant life cycle. Seed gene sets were compared with those expressed in prefertilization ovules, germinating seedlings, and leaves, roots, stems, and flora  ...[more]

PMID: 20385809

Publication: 1/2

Similar Datasets

Transcriptomics Expression data from Arabidopsis seed compartments at the mature green stage.
2009-03-12 | GSE15165 | GEO
Transcriptomics Transcription profiling of Arabidopsis thaliana seed compartments at the globular embryo stage.
2008-06-11 | E-GEOD-11262 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Arabidopsis seed compartments at the heart stage.
2009-03-21 | E-GEOD-15160 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Arabidopsis seed compartments at the pre-globular stage
2008-10-21 | E-GEOD-12402 | biostudies-arrayexpress
Transcriptomics Expression data from Arabidopsis seed compartments at the bending coyledon stage
2010-02-21 | E-GEOD-20039 | biostudies-arrayexpress
Transcriptomics Transcription profiling of Arabidopsis seed compartments at the linear-cotyledon stage
2008-10-21 | E-GEOD-12403 | biostudies-arrayexpress
Transcriptomics Expression data from Arabidopsis seed compartments at the linear-cotyledon stage
2008-08-12 | GSE12403 | GEO
Transcriptomics Expression data from Arabidopsis seed compartments at the heart stage.
2009-03-12 | GSE15160 | GEO
Transcriptomics Expression data from Arabidopsis Seed Compartments at the Globular Embryo Stage.
2008-04-25 | GSE11262 | GEO
Transcriptomics Expression data from Arabidopsis seed compartments at the pre-globular stage
2008-08-12 | GSE12402 | GEO