Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Gene expression during mouse OS25 ES cell differentiation

ABSTRACT: Position within chromosome territories, and localisation at transcription factories, are two facets of nuclear organisation that have been associated with active gene expression. However, there is still debate about whether this organisation is a cause or consequence of transcription. We induced looping out from chromosome territories (CTs), by the activation of Hox loci during differentiation, to investigate consequences on neighbouring loci. This microarray study aims at analyzing the expression of genes close to Hoxb and Hoxd clusters in parallel with their nuclear position. OS25 mouse ES cells were differentiated using retinoic acid in two independent experiments, and control undifferentiated cells were also cultured. For each experiment, labelled cDNAs were prepared from undifferentiated and differentiated cells and hybridized together on VUMC MACF Mouse 38K oligo v64 microarrays. Labelling dyes (Cy3 / Cy5) were swapped for cDNAs from the second differentiation experiment.

ORGANISM(S): Mus musculus

SUBMITTER: ClÃ©mence Kress

PROVIDER: E-GEOD-15166 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Lack of bystander activation shows that localization exterior to chromosome territories is not sufficient to up-regulate gene expression.

Morey Céline C Kress Clémence C Bickmore Wendy A WA

Genome research 20090423 7

Position within chromosome territories and localization at transcription factories are two facets of nuclear organization that have been associated with active gene expression. However, there is still debate about whether this organization is a cause or consequence of transcription. Here we induced looping out from chromosome territories (CTs), by the activation of Hox loci during differentiation, to investigate consequences on neighboring loci. We show that, even though flanking genes are caugh ...[more]

PMID: 19389823

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Dataset Information

Gene expression during mouse OS25 ES cell differentiation

Publications

Lack of bystander activation shows that localization exterior to chromosome territories is not sufficient to up-regulate gene expression.

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