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Transcription profiling of human umbilical vein endothelial cells (HUVEC) exposed to sheer stress and intraluminal pressure

ABSTRACT: Intact living conduit vessels (umbilical veins) were exposed to normal or high intraluminal pressure, or low or high shear stress in combination with a physiological level of the other force. We used a unique vascular ex vivo perfusion system. After six hours of perfusion endothelial cells were isolated from the stimulated vessels and RNA was extracted. RNA from 16 experiments from each stimulation were pooled and analyzed in duplicate DNA microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Per-Arne Svensson

PROVIDER: E-GEOD-1518 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Differential global gene expression response patterns of human endothelium exposed to shear stress and intraluminal pressure.

Andersson Maria M Karlsson Lena L Svensson Per-Arne PA Ulfhammer Erik E Ekman Mikael M Jernås Margareta M Carlsson Lena M S LM Jern Sverker S

Journal of vascular research 20050908 5

We investigated the global gene expression response of endothelium exposed to shear stress and intraluminal pressure and tested the hypothesis that the two biomechanical forces induce a differential gene expression response pattern. Intact living human conduit vessels (umbilical veins) were exposed to normal or high intraluminal pressure, or to low or high shear stress in combination with a physiological level of the other force in a unique vascular ex vivo perfusion system. Gene expression prof ...[more]

PMID: 16155357

Similar Datasets

Human endothelium exposed to shear stress and pressure

Project description:Intact living conduit vessels (umbilical veins) were exposed to normal or high intraluminal pressure, or low or high shear stress in combination with a physiological level of the other force. We used a unique vascular ex vivo perfusion system. After six hours of perfusion endothelial cells were isolated from the stimulated vessels and RNA was extracted. RNA from 16 experiments from each stimulation were pooled and analyzed in duplicate DNA microarrays. Keywords: repeat sample

2004-07-07 | GSE1518 | GEO

Sex-specific transcriptional differences between male and female human umbilical vein endothelial cells (HUVEC) after shear stress.

Project description:To assess whether there is a sex-specific response to stress, we subjected male and female HUVEC to laminar shear of 6 dyn/cm2 for 24 hours and analysed changes in gene expression. 6.7 % of all mRNAs were regulated by shear stress. Female HUVEC showed a more pronounced transcriptional response to shear than did their male counterparts. In addition to quantitative differences, a number of genes were regulated in the opposite direction between the two sexes by shear stress.

2014-12-31 | GSE52211 | GEO

HUVEC Gene Expression of Irradiated Human Umbilical Vein Endothelial Cells

Project description:Ionizing radiation has become common for diagnostic and therapeutic purpose. Since it affects the cells at genetic level, it becomes necessary to evaluate its affects especially on endothelial cells which comprises the blood vessels and thus contributes in migration of blood cells, nutrients, oxygen etc. Human Umbilical Vein Endothelial Cells (HUVECs) are major model system used in vitro to study the nature and behaviour of endothelial cells.

2020-09-30 | GSE108723 | GEO

Characterization of the transcriptional heterogeneity present within the rat lymphatic and blood vasculatures

Project description:The goal and objective of this study was to identify the transcriptional profiles differentiating the artery, vein, and lymphatic lineages in the adult rat vasculature with particular emphasis on the unique elements of the collecting lymphatic vessel transcriptome. A 2 x 3 experimental design was utilized in which parallel arteries, veins, and lymphatics from two different tissue beds were examined. The rat thoracic duct was selected as a large, post-nodal collecting lymphatic vessel that exhibits excellent conduit-type behavior while the rat mesenteric lymphatic was selected as a smaller, pre-nodal collecting lymphatic vessel that exhibits excellent pump behavior (see Gashev AA, et al. Microcirculation. 2004 Sep;11(6):477-92. [PMID: 15371129]). The axillary artery and vein were selected for comparison to the thoracic duct due to their similar anatomical position distal to the common junction of the lymphatic and venous vascular trees and represent a large artery and large vein, respectively. The mesentery artery and vein were selected for comparison to the mesenteric lymphatic vessels due to their parallel position within the mesenteric vasculature and represent a small atery and small vein, respectively. A 2 x 3, reference-based, experimental design was utilized consisting of both large (thoracic) and small (mesenteric) arteries, veins, and collecting lymphatic vessels for a total of 6 sample groups with n=6 biological replicates present in each group. All vessels acquired from the same donor animal have the same numerical label and were handled in parallel through all experimental steps. Each vessel sample RNA sample was amplified, labeled with Cy5, and compared to the same Rat Universal Reference RNA sample (Stratagene, La Jolla, CA) that was amplified and labeled with Cy3 dye. No dye swaps were utilized.

2012-06-22 | E-GEOD-17015 | biostudies-arrayexpress

Bentonite clay pressure vessels

Project description:Bentonite clay pressure vessels

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Microarray analysis of mesenteric arteries and veins in Crohn's disease compared to controls from non-IBD samples

Project description:Histology in the mesentery pointed to altered blood vessels. This experiment was designed to define the differences in gene expression in vessels from Crohn's disease versus controls. Crohn's disease was separately evaluated in inflamed (central disease) areas and in adjacent noninflamed areas. Laser capture microdissection was carried out on Carnoy's fixed mesenteric samples, comparing normal arteries or veins with Crohn's inflamed or nonifnlamed arteries or veins.

2015-03-05 | E-GEOD-66545 | biostudies-arrayexpress

Genome-wide DNA methylation profiles of 20 paired (normal and varicose) vein samples from the patients with varicose vein disease.

Project description:To identify genes involved in the formation of a pathological phenotype of varicose veins, we attempted to analyze the methylome of their constituent cells and compare it to the methylome of normal veins from the same patient. We identified a number of genes of extracellular matrix remodeling and cell-cell communication whose methylation status was significantly changed in disease condition.

2018-09-04 | GSE68319 | GEO

Genome-wide gene expression profiles of 14 paired (normal and varicose) vein samples from the patients with varicose vein disease.

Project description:To identify genes whose products are involved in the formation of a pathological phenotype of varicose veins, we attempted to analyze the transcriptome of their constituent cells and compare it to the transcriptome of normal veins from the same patient. We identified a number of genes of extracellular matrix remodeling and cell-cell communication significantly changing gene expression status in disease condition.

2018-09-04 | GSE68309 | GEO

CFTR deficiency modulates the activity of endothelial mechanosensitive channels TRPV4 and PIEZO1; and drives endothelial barrier failure.

Project description:Cystic fibrosis (CF) is a life-shortening genetic disease caused by a mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. Despite reports of CFTR expression on endothelial cells, pulmonary vascular perturbations, and perfusion deficit in CF patients, the mechanism of pulmonary vascular disease in CF remains unclear. Here, we describe loss of small pulmonary blood vessels in CF patients with severe lung disease. Using a vessel-on-a-chip model, we establish a shear stress-dependent mechanism of endothelial barrier failure in CF involving calcium-permeable mechanosensitive channel TRPV4. Furthermore, we demonstrate that CFTR deficiency downregulates the function of PIEZO1, another calcium-permeable mechanosensitive channel involved in angiogenesis and wound repair, and further exacerbates loss of small pulmonary blood vessels. We show that CFTR directly interacts with PIEZO1 and enhances its function, and that CFTR deficiency reduces PIEZO1 activity. Our study identifies key cellular targets to mitigate loss of small pulmonary blood vessels in CF.

2024-09-18 | GSE244214 | GEO

Global transcript analysis of quantitative trait loci (QTL) associated with meat tenderness in pigs

Project description:We sequenced mRNA from 32 muscles (n=16 semimembranosus,n=16 longisimus dorsi) of two pig breeds (PuÅawska and Polish Landrace), which are differing in firmness. The differences between pig groups with respect to shear force of coocked meat were significant and amounted to 64.88 N (P<0.01) in longisimus dorsi and 14.8 N (P<0.05) for semimembranosus. Examination of mRNA levels between pig breeds differing in shear force. Project ID: 217776 founded National Science Centre (NCN), title: Application of targeted next generation sequencing (NGS) in the identification of genetic markers associated with the pork quality

2015-12-06 | E-GEOD-75707 | biostudies-arrayexpress

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