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Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary

ABSTRACT: Here, we analyzed nine Drosophila piRNA pathway mutants for their impacts on both small RNA populations and the sub-cellular localization patterns of Piwi proteins. We find that distinct piRNA pathways with differing components function in ovarian germ and somatic cells. Keywords: Epigenetics 20 libraries were analyzed, with 9 being control samples (heterozygotes).

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Colin Malone

PROVIDER: E-GEOD-15186 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Specialized piRNA pathways act in germline and somatic tissues of the Drosophila ovary.

Malone Colin D CD Brennecke Julius J Dus Monica M Stark Alexander A McCombie W Richard WR Sachidanandam Ravi R Hannon Gregory J GJ

Cell 20090423 3

In Drosophila gonads, Piwi proteins and associated piRNAs collaborate with additional factors to form a small RNA-based immune system that silences mobile elements. Here, we analyzed nine Drosophila piRNA pathway mutants for their impacts on both small RNA populations and the subcellular localization patterns of Piwi proteins. We find that distinct piRNA pathways with differing components function in ovarian germ and somatic cells. In the soma, Piwi acts singularly with the conserved flamenco pi ...[more]

PMID: 19395010

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