Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

TGF or TNF Time series in ARPE19


ABSTRACT: Aberrant epithelial-mesenchymal transition (EMT) is involved in pathological processes including fibrotic disorders and cancer invasion and metastasis. Alterations of the cell-extracellular matrix (ECM) interaction also contribute to those pathological settings. However, the functional interplay between EMT and cell-ECM interaction is poorly understood. Here, we show that tumor necrosis factor (TNF)-alpha, a potent mediator of inflammation, induces EMT-associated fibrosis in retinal pigment epithelial cells, and that this is regulated by hyaluronan (HA)-CD44-Moesin interaction. TNF-alpha elicits both HA synthesis and Moesin phosphorylation through protein kinase C activation, promoting binding of CD44 to the newly synthesized HA. The HA-CD44-Moesin interaction leads to cell-cell dissociation through actin remodeling and increased cellular motility associated with mesenchymal phenotype. Furthermore, we established an in vivo model of TNF-alpha-induced fibrosis in the mouse eye, and the ocular fibrosis was completely suppressed in CD44-null mice. Therefore, HA production and its interaction with CD44 plays essential role in TNF-alpha-induced-EMT, and the interference of the complex formation can be a new strategy for the fibrotic disorders. ARPE19 cell lines were treated with TGF and TNF for 6 and 42 hour. Each experiment were repeated three times. But 1hour experiment was repeated two times. For this submission, total RNA was extracted from TGF- or TNF-treated ARPE-19 cells and differential gene expression between each time point (6 and 42 hours) was determined using genechip arrays (Affymetrix, Human Genome U133).

ORGANISM(S): Homo sapiens

SUBMITTER: Rui Ose 

PROVIDER: E-GEOD-15205 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2010-03-11 | GSE15205 | GEO
2023-05-22 | E-MTAB-11676 | biostudies-arrayexpress
2013-11-01 | E-GEOD-51938 | biostudies-arrayexpress
2021-12-13 | GSE164160 | GEO
2021-08-19 | PXD025821 | Pride
2024-10-15 | GSE266052 | GEO
2020-02-11 | GSE122305 | GEO
2008-06-12 | E-GEOD-2705 | biostudies-arrayexpress
2023-10-30 | PXD041505 | Pride
2015-03-07 | E-GEOD-66634 | biostudies-arrayexpress