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CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions


ABSTRACT: Plasmacytoid dendritic cells (pDCs) are key regulators of anti-viral immunity. They rapidly secrete IFN-alpha and cross-present viral antigens thereby launching adaptive immunity. Here we show that activated human pDCs inhibit replication of cancer cells, and kill them in a contact dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-alpha and express Granzyme B and TRAIL. CD2high pDCs uniquely express lysozyme and can be found in tonsils and in tumors. Both subsets launch recall T cell response. However, CD2high pDCs secrete higher levels of IL12 p40, express higher levels of co-stimulatory molecule CD80 and are more efficient in triggering proliferation of naïve allogeneic T cells. Thus, human blood pDCs are composed of subsets with specific phenotype and functions. pDC subsets were isolated from two different donors. They were analyzed in two independent experiments.

ORGANISM(S): Homo sapiens

SUBMITTER: Damien Chaussabel 

PROVIDER: E-GEOD-15215 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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CD2 distinguishes two subsets of human plasmacytoid dendritic cells with distinct phenotype and functions.

Matsui Toshimichi T   Connolly John E JE   Michnevitz Mark M   Chaussabel Damien D   Yu Chun-I CI   Glaser Casey C   Tindle Sasha S   Pypaert Marc M   Freitas Heidi H   Piqueras Bernard B   Banchereau Jacques J   Palucka A Karolina AK  

Journal of immunology (Baltimore, Md. : 1950) 20090601 11


Plasmacytoid dendritic cells (pDCs) are key regulators of antiviral immunity. They rapidly secrete IFN-alpha and cross-present viral Ags, thereby launching adaptive immunity. In this study, we show that activated human pDCs inhibit replication of cancer cells and kill them in a contact-dependent fashion. Expression of CD2 distinguishes two pDC subsets with distinct phenotype and function. Both subsets secrete IFN-alpha and express granzyme B and TRAIL. CD2(high) pDCs uniquely express lysozyme an  ...[more]

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