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Small RNAs in Arabidopsis hybrid siliques

ABSTRACT: Five days post-fertilization siliques were collected for RNA extraction. After purification, small RNA libraries were generated for high throughput sequencing. Resulting sequences were analyzed to determine the contribution of the paternal genome to the small RNA transcriptome. Keywords: Epigenetics 4 unique samples. small RNAs purified from Arabidopsis siliques 5 days after pollination. Samples were selfed or hybrid crosses between ecotypes Columbia (Col) and Landsberg erecta (Ler).

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Rebecca Mosher 

PROVIDER: E-GEOD-15348 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Uniparental expression of PolIV-dependent siRNAs in developing endosperm of Arabidopsis.

Mosher Rebecca A RA   Melnyk Charles W CW   Kelly Krystyna A KA   Dunn Ruth M RM   Studholme David J DJ   Baulcombe David C DC  

Nature 20090603 7252

Most eukaryotes produce small RNA (sRNA) mediators of gene silencing that bind to Argonaute proteins and guide them, by base pairing, to an RNA target. MicroRNAs (miRNAs) that normally target messenger RNAs for degradation or translational arrest are the best-understood class of sRNAs. However, in Arabidopsis thaliana flowers, miRNAs account for only 5% of the sRNA mass and less than 0.1% of the sequence complexity. The remaining sRNAs form a complex population of more than 100,000 different sma  ...[more]

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