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Gene expression analysis of dendritic cells treated with small chemical compounds

ABSTRACT: Identification of newer compounds to modulate dendritic cell functions. Total RNA obtained from bone marrow-derived dendritic cells treated for 6 hours with small chemical compounds or vehicle alone in the presence or absence of lipopolysaccharide (LPS).

ORGANISM(S): Mus musculus

SUBMITTER: Hironori Matsushima

PROVIDER: E-GEOD-15360 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Identification of crassin acetate as a new immunosuppressant triggering heme oxygenase-1 expression in dendritic cells.

Matsushima Hironori H Tanaka Hiroaki H Mizumoto Norikatsu N Takashima Akira A

Blood 20090428 1

By screening 720 natural compounds in a standard 2-way allogeneic mixed leukocyte reaction assay, we identified a potent immunosuppressive capacity of crassin acetate (CRA), a coral-derived cembrane diterpenoid. CRA efficiently inhibited allogeneic mixed leukocyte reaction as well as antigen-specific activation of CD4 T cells by bone marrow-derived dendritic cells (DCs). With regard to cellular targets, CRA suppressed not only mitogen-triggered T-cell activation, but also lipopolysaccharide-indu ...[more]

PMID: 19401559

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Gene expression analysis of dendritic cells treated with small chemical compounds

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Effect of cholera toxin or forskolin on mouse bone marrow-derived dendritic cells

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A dominant role for the methyl-CpG-binding protein Mbd2 in controlling Th2 induction by dendritic cells [gene expression]

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