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Metatranscriptomic analysis of an anaerobic ammonium oxidizing community dominated by Kuenenia stuttgartiensis

ABSTRACT: We compiled a metatranscriptome by extracting total RNA (including ribosomes), reverse transcription and solexa sequencing. We obtained quantitative data on the transcription of each orf to assess the importance of each orf to the metabolism of Kuenenia stuttgartiensis The transcriptomic data are combined with proteomic data and physiological/biochemical experiments

ORGANISM(S): Candidatus Kuenenia stuttgartiensis

SUBMITTER: Marc Strous

PROVIDER: E-GEOD-15408 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Molecular mechanism of anaerobic ammonium oxidation.

Kartal Boran B Maalcke Wouter J WJ de Almeida Naomi M NM Cirpus Irina I Gloerich Jolein J Geerts Wim W Op den Camp Huub J M HJ Harhangi Harry R HR Janssen-Megens Eva M EM Francoijs Kees-Jan KJ Stunnenberg Hendrik G HG Keltjens Jan T JT Jetten Mike S M MS Strous Marc M

Nature 20111002 7371

Two distinct microbial processes, denitrification and anaerobic ammonium oxidation (anammox), are responsible for the release of fixed nitrogen as dinitrogen gas (N(2)) to the atmosphere. Denitrification has been studied for over 100 years and its intermediates and enzymes are well known. Even though anammox is a key biogeochemical process of equal importance, its molecular mechanism is unknown, but it was proposed to proceed through hydrazine (N(2)H(4)). Here we show that N(2)H(4) is produced f ...[more]

PMID: 21964329

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