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Expression data from human embryonic kidney cells (HEK293) cultivated in high and low glucose

ABSTRACT: Gene expression profiling of human embryonic kidney (HEK293) cells was performed to determine the effect of high and low glucose on gene expression. Microarrays were used to identify distinct classes of genes up-regulated in HEK293 cells during cultivation for 7 days in medium with physiological (low) glucose compared to high glucose. Human embryonic kidney cells (HEK293) were cultivated for 7 days in commercially available DMEM (supplemented with 10 % FCS) containing high glucose (450 mg/dl) or low glucose (100 mg/dl). Cells were harvested, total RNA was extracted and microarray gene expression profiling was performed to compare differential gene expression between low and high glucose conditions. Two biological replicates for each condition were made (high glucose: HEK-high-gluc-1 and HEK-high-gluc-2; and low glucose: HEK-low-gluc-1 and HEK-low-gluc-2).

ORGANISM(S): Homo sapiens

SUBMITTER: Ursula Quitterer

PROVIDER: E-GEOD-15575 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Calreticulin enhances B2 bradykinin receptor maturation and heterodimerization.

Abd Alla Joshua J Reeck Kristin K Langer Andreas A Streichert Thomas T Quitterer Ursula U

Biochemical and biophysical research communications 20090704 1

In different native tissues and cells the receptor for the vasodepressor bradykinin, B(2), forms dimers with the receptor for the vasopressor angiotensin II, AT(1). Because AT(1)/B(2) heterodimers may contribute to enhanced angiotensin II-stimulated signaling under pathophysiological conditions, we analyzed mechanisms of AT(1)/B(2) heterodimerization. We found that efficient B(2) receptor maturation was a prerequisite for heterodimerization because only the fully mature B(2) receptor was capable ...[more]

PMID: 19580784

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