Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

44 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription profiling of rat freshly-isolated alveolar epithelial type I cells, freshly-isolated type II cells, and type II cells cultured for 7 days

ABSTRACT: Comparison of rat freshly-isolated alveolar epithelial type I cells, freshly-isolated type II cells, and type II cells cultured for 7 days

ORGANISM(S): Rattus norvegicus

SUBMITTER: Leland Dobbs

PROVIDER: E-GEOD-1567 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Freshly isolated rat alveolar type I cells, type II cells, and cultured type II cells have distinct molecular phenotypes.

Gonzalez Robert R Yang Yee Hwa YH Griffin Chandi C Allen Lennell L Tigue Zachary Z Dobbs Leland L

American journal of physiology. Lung cellular and molecular physiology 20040924 1

We used microarray analysis with Affymetrix rat chips to determine gene expression profiles of freshly isolated rat type I (TI) and TII cells and cultured TII cells. Our goals were 1) to describe molecular phenotypic "fingerprints" of TI and TII cells, 2) to gain insight into possible functional differences between the two cell types through differentially expressed genes, 3) to identify genes that might indicate potential functions of TI cells, since so little is known about this cell type, and ...[more]

PMID: 15447939

Similar Datasets

Transcription profiling of human fetal lung epithelial cells in undifferentiated state and after type II cell differentiation induced by DCI treatment

Project description:Studies of human fetal lung in explant culture and in isolated epithelial cells have demonstrated that both glucocorticoids and cyclic AMP promote differentiated alveolar type II cell phenotype as assessed by ultrastructural morphology and surfactant production. This project profiles changes in gene expression associated with hormone induced differentiation. Undifferentiated human fetal lung (13-20 wk) epithelial cells were cultured in serum-free medium (control) or with dexamethasone/8-Bromo cyclic AMP/isobutylmethylxanthine (DCI) to promote type II cell differentiation. RNA from five sets of experiments (10 samples) was evaluated using the U133A Affymetrix GeneChip set. Experiment Overall Design: Cells were isolated from 13 individual lungs and the cells from each were cultured in the absence (control) and presence of DCI dexamethasone (10 nM)/8-Br-cyclic AMP (0.1 mM)/isobutylxanthine for 72 h. RNA was isolated from the control and treated cells of each prep. For the 5 sets of microarray experiments (10 total chips, 2 for each control and treated pair), RNA from 2 of the cell preps was analysed as individual microarray comparisons. RNA from 11 cell preps was pooled for the other 3 experiments (4, 4, and 3 cell preps) to provide control and DCI-treated RNA pools.

2007-11-22 | E-GEOD-3306 | biostudies-arrayexpress

Transcription profiling of mouse alveolar macrophage from animals with mild or severe asthma

Project description:Th2, Th1 and Th17 biased sensitization against ovalbumin was achieved by immunization with Complete Freunds Adjuvant as opposed to alum-promoted Th2 biased sensitization. Bronchial inflammation was elicited by ovalbumin inhalation. T-cell, granulocyte and inflammatory mediator profiles in BAL and lungs were determined along with alveolar macrophage transcriptome profiling.

2010-06-30 | E-MEXP-2500 | biostudies-arrayexpress

Transcription profiling of human bronchial epithelial cells treated with vehicle (control), VOSO4 (V, 50 uM) or ZnSO4 (Zn, 50 uM) for 4 hours.

Project description:Human bronchial epithelial cells were treated with vehicle (control), VOSO4 (V, 50 uM) or ZnSO4 (Zn, 50 uM) for 4 hours.

2007-08-25 | E-GEOD-2111 | biostudies-arrayexpress

Transcription profiling of rat duodenal samples at different developmental stages and the effects of iron-deficiency

Project description:The effect of iron-deficiency on duodenal gene expression was investigated in rats at different developmental stages.

2007-09-15 | E-GEOD-1892 | biostudies-arrayexpress

Transcription profiling of irradiated human dermal microvascular endothelial cells

Project description:Human dermal microvascular endothelial cells (HDMVECs) were irradiated with 2 Gy or non-irradiated. Two methods of RNA isolation were used to comparatively analyze the miRNA status after radiation treatment.

2010-01-13 | E-TABM-617 | biostudies-arrayexpress

Transcription profiling of liver from wild type and tumor suppressor Apc knock-out mice

Project description:A "Cartes d'Identite des Tumeurs" (CIT) project from the french Ligue Nationale Contre le Cancer (http://cit.ligue-cancer.net). This work aims to demonstrate a key role for the Wnt/beta-catenin pathway in liver embryonic growth and in controlling the fate of hepatoblasts, preventing them to differentiate towards the hepatocyte lineage, and guiding them to a duct morphogenesis. Through the inactivation of Apc (adenomatous Polyposis Coli) tumor suppressor gene in hepatoblasts by a Cre-loxP strategy, the ectopic activation of beta-catenin targeted in hepatoblasts after liver bud formation leads to a lethal embryonic phenotype, characterized by liver hypoplasia, a blockade of hepatocyte differentiation and commitment to a biliary fate. (this work was supported by INSERM and the B^SLigue Nationale

2007-08-07 | E-TABM-295 | biostudies-arrayexpress

Transcription profiling of yeast TOP1 null strain bearing plasmids that express wild-type yeast Top1p or an inactive Y727F mutant enzyme and treated with camptothecin

Project description:To establish the effect of CPT in the regulation of global transcription in S. cerevisiae, we have used a yeast TOP1 null strain, JEL1Δtop1, bearing a low-copy number plasmid that expresses, under the control of the yeast TOP1 promoter, either a wild-type (wt) yeast Top1p (pCC10) or an inactive Y727F mutant enzyme (pAR7).We determined global transcript levels upon CPT treatments of JEL1Δtop1 cells expressing either a yeast wt or Y727F mutant Top1p.

2008-06-01 | E-MEXP-1104 | biostudies-arrayexpress

Transcription profiling time series of mouse bone marrow derived macrophages after interferon-gamma treatment

Project description:We have examined the transcriptional events in mouse bone marrow derived macrophages (MBDM) with interferon-gamma (Ifng)at 2, 4 & 8 h following treatment or pre-treatment (0 h).

2008-03-18 | E-MEXP-1490 | biostudies-arrayexpress

Ribonucleoprotein immunoprecipitation of fission yeast to study myosin proteins and myosin-specific chaperones

Project description:Ribonucleoprotein immunoprecipitation microarray (RIp-chip) study using various myosin proteins and myosin-specific chaperones from the fission Schizosaccaromyces pombe. Two strains were used. In rng3TAP the rng3 protein has been tagged with TAP to allow its detection and immunoprecipitation. The TAP strain expresses the TAP sequence alone (without being attached to an other protein)

2008-12-23 | E-TABM-516 | biostudies-arrayexpress

Transcription profiling of mouse lung fibroblasts and Lewis lung cells to investigate the transciptional regulation that governs cell-cell fusion process

Project description:This study aimed to investigate the transciptional regulation that governs cell-cell fusion process.

2008-06-30 | E-TABM-381 | biostudies-arrayexpress