Browse
Submit Data
Databases
API
Help

Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression-based dentification of microRNA-9-3 target genes in breast epithelial cells

ABSTRACT: In current study, DES could effectively suppress the expression of miR-9-3 through the ERa-dependent epigenetic machinery in MDECs and MCF-7 cells. We want to figure out the biological significance and the potential targets of miR-9-3 and the related signaling cascades. MCF-7 cells were transfected with two pre-designed maturated mimics of miR-9-3, miR-9 and miR-9*. After 48hr of transfection, total RNA was collected for gene expression analysis to figure out the potential targets of miR-9-3.

ORGANISM(S): Homo sapiens

SUBMITTER: Pei-Yin Hsu

PROVIDER: E-GEOD-15749 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Similar Datasets

Gene expression-based dentification of microRNA-9-3 target genes in breast epithelial cells

Project description:In current study, DES could effectively suppress the expression of miR-9-3 through the ERa-dependent epigenetic machinery in MDECs and MCF-7 cells. We want to figure out the biological significance and the potential targets of miR-9-3 and the related signaling cascades.

2011-12-16 | GSE15749 | GEO

Genomic binding profiles of ERa in MCF-7 and T47D control or enhancer588-targeted cells

Project description:Estrogen Receptor a (ERa) bindning to DNA was profiled by ChIP-seq in MCF-7 and T47D cells transduced with either control sgRNA, or sgRNA targeting a specific enhancer region (enhancer588). ERa in MCF-7 and T47D control or enhancer588-targeted cells

2015-12-14 | E-GEOD-75779 | biostudies-arrayexpress

Expression data from mir-429 transfected HEY cells in a time-point series (0hr-HEY, 24hrs, 48hrs and 144hrs)

Project description:HEY cells display a mesenchymal phenotype (elongated). After a 48hr transfection with mir-429 their phenotype changes to an epithelial-like (cuboidal)

2018-02-27 | GSE111139 | GEO

Quantitative Analysis of Wild Type and scrib mutant Drosophila wing imaginal discs Transcriptomes at different time points

Project description:Purpose: There are two goals of this study. Firstly, we want to figure out the transcriptomic differences between control and scrib¹ mutant wing imaginal discs. Secondly, we aim to investigate the transcriptomic changes as the scrib¹ mutant tumor develop over time. Methods: Total RNA was used to generate cDNA libraries and 150bp paired-end sequencing on Illumina HiSeq platform were performed by Novogene. Cleaned raw reads were mapped to the reference genome using STAR and counts are generated by featuresCounts available in Subread package. Count normalization was performed using edgeR.

2019-08-14 | GSE130243 | GEO

miRNA expression data from MCF-7 cells, overexpressing ANXA1

Project description:Annexin 1 (ANXA1), an endogenous anti-inflammatory protein which modulates cellular processes such as proliferation, differentiation and apoptosis has been implicated in cancer initiation and progression. ANXA1 was previously shown to be regulated by hsa-miR-196a and promoted cell proliferation and anchorarge-dependent growth and suppressed apoptosis. However, whether ANXA1 itself regulates miRNA expression is unknown. Therefore, in this study, we investigated the regulation of miRNA by ANXA1 in breast cancer cells. Using microarray technology, 12 miRNAs were found to be significantly and consistently downregulated in MCF-7 cells (MCF-V5) overexpressing ANXA1 overexpressing MCF-7 cells (MCF-V5). Hsa-miR-26b* and hsa-miR-562 were chosen for further investigation.The data suggest that miR-26b* and miR-562 may play a role in ANXA1-induced migration and possibly angiogenesis by targeting NFKB and point towards a potential therapeutic target for breast cancer. Breast cancer MCF-7 cells (MCF-V5) overexpressing ANXA1 were cultured for RNA extraction and hybridization on Affymetrix miRNA microarrays. These were compared against the control, which were MCF-7 cells (MCF-EV) carrying an empty expression vector. Expression analyses were carried out in triplicates

2015-01-05 | E-GEOD-54439 | biostudies-arrayexpress

RNA-seq in breast cancer cell lines after manipulation of miR-23b expression

Project description:RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-23b in both MCF-7 and MDA-MB-231 breast cancer cell lines

2013-05-01 | GSE37918 | GEO

RNA-sequencing (RNA-seq) in breast cancer cell lines after ectopic manipulation of miR-26a expression

Project description:RNA sequencing technology has been carried out in order to evaluate mRNA expression changes after manipulation of miR-26a in both MCF-7 and MDA-MB-231 breast cancer cell lines.

2016-06-30 | GSE43726 | GEO

miRNA Lisbon

Project description:Proteomic analysis of differentially expressed proteins in MDA-MB-231 and MCF-10A cell lines when miR-200c and miR-203 were transiently expressed or inhibited, respectively.

2022-02-28 | PXD007210 | Pride

Genomic binding profiles of ERa in MCF-7 and T47D control or enhancer588-targeted cells

2015-12-14 | GSE75779 | GEO

Estrogen-responsive Estrogen Receptor alpha binding sites in MCF-7 by ChIP-seq

Project description:Identification of Estrogen Receptor alpha (ERa) binding sites by ChIP-seq in MCF-7 breast cancer cells following an estrogen treatment. This study describes molecular effects of estradiol treatment and subsequent regulation by ER for a single gene/locus. A public ER chipseq (available in SRA as ERR011973), in addition to our own data, guided us to regulatory regions were ER was binding that were then analyzed in detail using "manual" ChIP. MCF-7 cells were treated for 1 h either 10 nm estradiol (E2) or vehicle (ethanol) and subjected to ChIP using antibodies against ERa or IgG.

2012-12-17 | E-GEOD-34759 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data